ID   TUSE_ECOLI              Reviewed;         109 AA.
AC   P0AB18; P45572; P75878;
DT   11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Sulfurtransferase TusE;
DE            EC=2.8.1.-;
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein E;
GN   Name=tusE; Synonyms=yccK; OrderedLocusNames=b0969, JW0952;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-55.
RX   PubMed=6376117; DOI=10.1002/j.1460-2075.1984.tb01936.x;
RA   Tamura F., Nishimura S., Ohki M.;
RT   "The E. coli divE mutation, which differentially inhibits synthesis of
RT   certain proteins, is in tRNASer1.";
RL   EMBO J. 3:1103-1107(1984).
RN   [5]
RP   IDENTIFICATION.
RX   PubMed=7567469; DOI=10.1093/nar/23.17.3554;
RA   Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., Danchin A.;
RT   "Detection of new genes in a bacterial genome using Markov models for three
RT   gene classes.";
RL   Nucleic Acids Res. 23:3554-3562(1995).
RN   [6]
RP   FUNCTION, MUTAGENESIS OF CYS-108, AND SUBUNIT.
RX   PubMed=16387657; DOI=10.1016/j.molcel.2005.11.001;
RA   Ikeuchi Y., Shigi N., Kato J., Nishimura A., Suzuki T.;
RT   "Mechanistic insights into sulfur relay by multiple sulfur mediators
RT   involved in thiouridine biosynthesis at tRNA wobble positions.";
RL   Mol. Cell 21:97-108(2006).
CC   -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC       Could accept sulfur from TusD. {ECO:0000269|PubMed:16387657}.
CC   -!- SUBUNIT: Interacts with the TusBCD complex. Interacts with MnmA.
CC       {ECO:0000269|PubMed:16387657}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DsrC/TusE family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=X00547; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; U00096; AAC74055.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35734.1; -; Genomic_DNA.
DR   EMBL; X00547; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; H64837; H64837.
DR   RefSeq; NP_415489.4; NC_000913.3.
DR   RefSeq; WP_000904442.1; NZ_STEB01000006.1.
DR   AlphaFoldDB; P0AB18; -.
DR   SMR; P0AB18; -.
DR   BioGRID; 4260042; 10.
DR   BioGRID; 849414; 2.
DR   ComplexPortal; CPX-2144; TusBCDE complex.
DR   ComplexPortal; CPX-2145; tRNA-specific 2-thiouridylase tusE-mnmA complex.
DR   DIP; DIP-48154N; -.
DR   IntAct; P0AB18; 11.
DR   STRING; 511145.b0969; -.
DR   jPOST; P0AB18; -.
DR   PaxDb; P0AB18; -.
DR   PRIDE; P0AB18; -.
DR   EnsemblBacteria; AAC74055; AAC74055; b0969.
DR   EnsemblBacteria; BAA35734; BAA35734; BAA35734.
DR   GeneID; 66670755; -.
DR   GeneID; 945023; -.
DR   KEGG; ecj:JW0952; -.
DR   KEGG; eco:b0969; -.
DR   PATRIC; fig|1411691.4.peg.1304; -.
DR   EchoBASE; EB2714; -.
DR   eggNOG; COG2920; Bacteria.
DR   HOGENOM; CLU_153199_1_0_6; -.
DR   InParanoid; P0AB18; -.
DR   OMA; EYNTSPA; -.
DR   PhylomeDB; P0AB18; -.
DR   BioCyc; EcoCyc:EG12876-MON; -.
DR   BioCyc; MetaCyc:EG12876-MON; -.
DR   PRO; PR:P0AB18; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:1990228; C:sulfurtransferase complex; IPI:ComplexPortal.
DR   GO; GO:0097163; F:sulfur carrier activity; IDA:EcoCyc.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IDA:ComplexPortal.
DR   Gene3D; 1.10.10.370; -; 1.
DR   Gene3D; 3.30.1420.10; -; 1.
DR   InterPro; IPR042072; DsrC-like_C.
DR   InterPro; IPR025526; DsrC-like_dom_sf.
DR   InterPro; IPR043163; DsrC-like_N.
DR   InterPro; IPR007453; DsrC/TusE.
DR   PANTHER; PTHR37010; PTHR37010; 1.
DR   Pfam; PF04358; DsrC; 1.
DR   PIRSF; PIRSF006223; DsrC_TusE; 1.
DR   SUPFAM; SSF69721; SSF69721; 1.
DR   TIGRFAMs; TIGR03342; dsrC_tusE_dsvC; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Reference proteome; Transferase; tRNA processing.
FT   CHAIN           1..109
FT                   /note="Sulfurtransferase TusE"
FT                   /id="PRO_0000168789"
FT   ACT_SITE        108
FT                   /note="Cysteine persulfide intermediate"
FT   MUTAGEN         108
FT                   /note="C->S: Loss of activity."
FT                   /evidence="ECO:0000269|PubMed:16387657"
SQ   SEQUENCE   109 AA;  12410 MW;  FDB0CFF89114EBE7 CRC64;
     MLIFEGKEIE TDTEGYLKES SQWSEPLAVV IAENEGISLS PEHWEVVRFV RDFYLEFNTS
     PAIRMLVKAM ANKFGEEKGN SRYLYRLFPK GPAKQATKIA GLPKPVKCI