ID   TUSE_SHIFL              Reviewed;         109 AA.
AC   P0AB19; P45572; P75878;
DT   11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Sulfurtransferase TusE;
DE            EC=2.8.1.-;
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein E;
GN   Name=tusE; OrderedLocusNames=SF0971, S1037;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC       Could accept sulfur from TusD (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with the TusBCD complex. Interacts with MnmA (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DsrC/TusE family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN42599.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAP16485.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE005674; AAN42599.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AE014073; AAP16485.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_706892.3; NC_004337.2.
DR   RefSeq; WP_000904442.1; NZ_WPGW01000043.1.
DR   AlphaFoldDB; P0AB19; -.
DR   SMR; P0AB19; -.
DR   STRING; 198214.SF0971; -.
DR   EnsemblBacteria; AAN42599; AAN42599; SF0971.
DR   EnsemblBacteria; AAP16485; AAP16485; S1037.
DR   GeneID; 1026221; -.
DR   GeneID; 66670755; -.
DR   KEGG; sfl:SF0971; -.
DR   KEGG; sfx:S1037; -.
DR   PATRIC; fig|198214.7.peg.1130; -.
DR   HOGENOM; CLU_153199_0_0_6; -.
DR   OrthoDB; 1840461at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.370; -; 1.
DR   Gene3D; 3.30.1420.10; -; 1.
DR   InterPro; IPR042072; DsrC-like_C.
DR   InterPro; IPR025526; DsrC-like_dom_sf.
DR   InterPro; IPR043163; DsrC-like_N.
DR   InterPro; IPR007453; DsrC/TusE.
DR   PANTHER; PTHR37010; PTHR37010; 1.
DR   Pfam; PF04358; DsrC; 1.
DR   PIRSF; PIRSF006223; DsrC_TusE; 1.
DR   SUPFAM; SSF69721; SSF69721; 1.
DR   TIGRFAMs; TIGR03342; dsrC_tusE_dsvC; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; Transferase; tRNA processing.
FT   CHAIN           1..109
FT                   /note="Sulfurtransferase TusE"
FT                   /id="PRO_0000168790"
FT   ACT_SITE        108
FT                   /note="Cysteine persulfide intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   109 AA;  12410 MW;  FDB0CFF89114EBE7 CRC64;
     MLIFEGKEIE TDTEGYLKES SQWSEPLAVV IAENEGISLS PEHWEVVRFV RDFYLEFNTS
     PAIRMLVKAM ANKFGEEKGN SRYLYRLFPK GPAKQATKIA GLPKPVKCI