ID   TUSE_YERPE              Reviewed;         109 AA.
AC   Q8ZG65; Q0WGX0; Q74VH1; Q8D060;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 2.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=Sulfurtransferase TusE;
DE            EC=2.8.1.-;
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein E;
GN   Name=tusE; OrderedLocusNames=YPO1447, y2723, YP_1338;
OS   Yersinia pestis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=632;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CO-92 / Biovar Orientalis;
RX   PubMed=11586360; DOI=10.1038/35097083;
RA   Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA   Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA   Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA   Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA   Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA   Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA   Stevens K., Whitehead S., Barrell B.G.;
RT   "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL   Nature 413:523-527(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KIM10+ / Biovar Mediaevalis;
RX   PubMed=12142430; DOI=10.1128/jb.184.16.4601-4611.2002;
RA   Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA   Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D.,
RA   Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A.,
RA   Nilles M.L., Matson J.S., Blattner F.R., Perry R.D.;
RT   "Genome sequence of Yersinia pestis KIM.";
RL   J. Bacteriol. 184:4601-4611(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=91001 / Biovar Mediaevalis;
RX   PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA   Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA   Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA   Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA   Wang J., Huang P., Yang R.;
RT   "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT   avirulent to humans.";
RL   DNA Res. 11:179-197(2004).
CC   -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC       Could accept sulfur from TusD (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with the TusBCD complex. Interacts with MnmA (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DsrC/TusE family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAL20098.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AL590842; CAL20098.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AE009952; AAM86275.1; -; Genomic_DNA.
DR   EMBL; AE017042; AAS61581.1; -; Genomic_DNA.
DR   PIR; AH0176; AH0176.
DR   RefSeq; WP_002213046.1; NZ_WUCM01000066.1.
DR   RefSeq; YP_002346468.1; NC_003143.1.
DR   AlphaFoldDB; Q8ZG65; -.
DR   SMR; Q8ZG65; -.
DR   STRING; 214092.YPO1447; -.
DR   PaxDb; Q8ZG65; -.
DR   DNASU; 1147670; -.
DR   EnsemblBacteria; AAM86275; AAM86275; y2723.
DR   EnsemblBacteria; AAS61581; AAS61581; YP_1338.
DR   GeneID; 66842100; -.
DR   KEGG; ype:YPO1447; -.
DR   KEGG; ypk:y2723; -.
DR   KEGG; ypm:YP_1338; -.
DR   PATRIC; fig|214092.21.peg.1773; -.
DR   eggNOG; COG2920; Bacteria.
DR   HOGENOM; CLU_153199_1_0_6; -.
DR   OMA; EYNTSPA; -.
DR   Proteomes; UP000000815; Chromosome.
DR   Proteomes; UP000001019; Chromosome.
DR   Proteomes; UP000002490; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0097163; F:sulfur carrier activity; IBA:GO_Central.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IBA:GO_Central.
DR   Gene3D; 1.10.10.370; -; 1.
DR   Gene3D; 3.30.1420.10; -; 1.
DR   InterPro; IPR042072; DsrC-like_C.
DR   InterPro; IPR025526; DsrC-like_dom_sf.
DR   InterPro; IPR043163; DsrC-like_N.
DR   InterPro; IPR007453; DsrC/TusE.
DR   PANTHER; PTHR37010; PTHR37010; 1.
DR   Pfam; PF04358; DsrC; 1.
DR   PIRSF; PIRSF006223; DsrC_TusE; 1.
DR   SUPFAM; SSF69721; SSF69721; 1.
DR   TIGRFAMs; TIGR03342; dsrC_tusE_dsvC; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; Transferase; tRNA processing.
FT   CHAIN           1..109
FT                   /note="Sulfurtransferase TusE"
FT                   /id="PRO_0000234622"
FT   ACT_SITE        108
FT                   /note="Cysteine persulfide intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   109 AA;  12394 MW;  7362F90738632623 CRC64;
     MLEFEGRIID TDAQGYLKNS TDWSEALAPV LAEQEGITLT EPHWEVVRFV RAFYQEFNTS
     PAIRMLVKAM AQKYGEEKGN SRYLYRLFPK GPAKQATKIA GLPKPVKCI