ID   TUSE_YERPS              Reviewed;         108 AA.
AC   Q66CD8;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Sulfurtransferase TusE;
DE            EC=2.8.1.-;
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein E;
GN   Name=tusE; OrderedLocusNames=YPTB1465;
OS   Yersinia pseudotuberculosis serotype I (strain IP32953).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=273123;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IP32953;
RX   PubMed=15358858; DOI=10.1073/pnas.0404012101;
RA   Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O.,
RA   Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L.,
RA   Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C.,
RA   Simonet M., Chenal-Francisque V., Souza B., Dacheux D., Elliott J.M.,
RA   Derbise A., Hauser L.J., Garcia E.;
RT   "Insights into the evolution of Yersinia pestis through whole-genome
RT   comparison with Yersinia pseudotuberculosis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004).
CC   -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC       Could accept sulfur from TusD (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with the TusBCD complex. Interacts with MnmA (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DsrC/TusE family. {ECO:0000305}.
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DR   EMBL; BX936398; CAH20705.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q66CD8; -.
DR   SMR; Q66CD8; -.
DR   EnsemblBacteria; CAH20705; CAH20705; YPTB1465.
DR   KEGG; yps:YPTB1465; -.
DR   OMA; EYNTSPA; -.
DR   Proteomes; UP000001011; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.370; -; 1.
DR   Gene3D; 3.30.1420.10; -; 1.
DR   InterPro; IPR042072; DsrC-like_C.
DR   InterPro; IPR025526; DsrC-like_dom_sf.
DR   InterPro; IPR043163; DsrC-like_N.
DR   InterPro; IPR007453; DsrC/TusE.
DR   PANTHER; PTHR37010; PTHR37010; 1.
DR   Pfam; PF04358; DsrC; 1.
DR   PIRSF; PIRSF006223; DsrC_TusE; 1.
DR   SUPFAM; SSF69721; SSF69721; 1.
DR   TIGRFAMs; TIGR03342; dsrC_tusE_dsvC; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Transferase; tRNA processing.
FT   CHAIN           1..108
FT                   /note="Sulfurtransferase TusE"
FT                   /id="PRO_0000234623"
FT   ACT_SITE        107
FT                   /note="Cysteine persulfide intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   108 AA;  12281 MW;  480EF1E402E284A9 CRC64;
     MEFEGRIIDT DAQGYLKNST DWSEALAPVL AEQEGITLTE PHWEVVRFVR AFYQEFNTSP
     AIRMLVKAMA QKYGEEKGNS RYLYRLFPKG PAKQATKIAG LPKPVKCI