TVOK_THEVO
ID TVOK_THEVO Reviewed; 348 AA.
AC Q979Z2;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Calcium-gated potassium channel TvoK {ECO:0000305};
GN Name=tvoK {ECO:0000303|PubMed:17588939};
GN ORFNames=TVG1041141 {ECO:0000312|EMBL:BAB60160.1};
OS Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC
OS 15438 / GSS1).
OC Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC Thermoplasmataceae; Thermoplasma.
OX NCBI_TaxID=273116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1;
RX PubMed=11121031; DOI=10.1073/pnas.97.26.14257;
RA Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y.,
RA Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T.,
RA Yamamoto Y., Aramaki H., Makino K., Suzuki M.;
RT "Archaeal adaptation to higher temperatures revealed by genomic sequence of
RT Thermoplasma volcanium.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000).
RN [2]
RP FUNCTION, SUBUNIT, ALTERNATIVE INITIATION, AND MUTAGENESIS OF ARG-111;
RP LYS-114; LEU-115 AND LYS-117.
RX PubMed=17588939; DOI=10.1074/jbc.m703650200;
RA Parfenova L.V., Abarca-Heidemann K., Crane B.M., Rothberg B.S.;
RT "Molecular architecture and divalent cation activation of TvoK, a
RT prokaryotic potassium channel.";
RL J. Biol. Chem. 282:24302-24309(2007).
CC -!- FUNCTION: Calcium-gated potassium channel. Can also be activated by
CC Mg(2+), Mn(2+) and Ni(2+). {ECO:0000269|PubMed:17588939}.
CC -!- SUBUNIT: Heterooctamer composed of four full-length subunits and four
CC soluble RCK domains. {ECO:0000269|PubMed:17588939}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=1 {ECO:0000305}; Synonyms=Calcium-gated potassium channel
CC {ECO:0000305};
CC IsoId=Q979Z2-1; Sequence=Displayed;
CC Name=2 {ECO:0000305}; Synonyms=Soluble RCK domain {ECO:0000305};
CC IsoId=Q979Z2-2; Sequence=VSP_059160, VSP_059161;
CC -!- MISCELLANEOUS: [Isoform 2]: Starts at UUG codon.
CC {ECO:0000269|PubMed:17588939}.
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DR EMBL; BA000011; BAB60160.1; -; Genomic_DNA.
DR RefSeq; WP_010917246.1; NC_002689.2.
DR AlphaFoldDB; Q979Z2; -.
DR SMR; Q979Z2; -.
DR STRING; 273116.14325256; -.
DR TCDB; 1.A.1.13.3; the voltage-gated ion channel (vic) superfamily.
DR EnsemblBacteria; BAB60160; BAB60160; BAB60160.
DR GeneID; 1441128; -.
DR KEGG; tvo:TVG1041141; -.
DR eggNOG; arCOG01958; Archaea.
DR HOGENOM; CLU_050982_1_1_2; -.
DR OMA; HIIVCGC; -.
DR OrthoDB; 80000at2157; -.
DR PhylomeDB; Q979Z2; -.
DR Proteomes; UP000001017; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005267; F:potassium channel activity; IEA:UniProtKB-KW.
DR InterPro; IPR003280; 2pore_dom_K_chnl.
DR InterPro; IPR013099; K_chnl_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR003148; RCK_N.
DR Pfam; PF07885; Ion_trans_2; 1.
DR Pfam; PF02254; TrkA_N; 1.
DR PRINTS; PR01333; 2POREKCHANEL.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS51202; RCK_C; 1.
DR PROSITE; PS51201; RCK_N; 1.
PE 1: Evidence at protein level;
KW Alternative initiation; Calcium; Cell membrane; Ion channel; Ion transport;
KW Magnesium; Manganese; Membrane; Metal-binding; Nickel; Potassium;
KW Potassium channel; Potassium transport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..348
FT /note="Calcium-gated potassium channel TvoK"
FT /id="PRO_0000439237"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 122..253
FT /note="RCK N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00543"
FT DOMAIN 266..348
FT /note="RCK C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
FT VAR_SEQ 1..114
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000269|PubMed:17588939"
FT /id="VSP_059160"
FT VAR_SEQ 115
FT /note="L -> M (in isoform 2)"
FT /id="VSP_059161"
FT MUTAGEN 111
FT /note="R->N: Reduces levels of the soluble RCK domain."
FT /evidence="ECO:0000269|PubMed:17588939"
FT MUTAGEN 114
FT /note="K->N: Does not affect levels of the soluble RCK
FT domain."
FT /evidence="ECO:0000269|PubMed:17588939"
FT MUTAGEN 115
FT /note="L->N: Abolishes expression of the soluble RCK
FT domain."
FT /evidence="ECO:0000269|PubMed:17588939"
FT MUTAGEN 117
FT /note="K->N: Does not affect levels of the soluble RCK
FT domain."
FT /evidence="ECO:0000269|PubMed:17588939"
SQ SEQUENCE 348 AA; 38605 MW; 706E08967EABA376 CRC64;
MRPFRTLVKV LQKIESSPLT KVFMAFIIVV LIGSYLEFLT QRNVKYSEIK NYFTAIWFTM
ETVTTVGYGD VVPVSNLGRV VAMLIMVSGI GLLGTLTATI SAYLFQIRIE RRGKLEKRLK
NHTIICNWNA YTRNIIEGNR DEEAAPIVIL SENTDNSEKY QNVFFVKGDC TSEDDLKNAA
IEDAARVVIM SDIENNAIPE DLLDAKTLLS IFTVRKLNNA VEIIAEVRDE KNKKHAVSAG
ATEVLSVGEL SSRLISRSIS NPGLTDFILK SLSETEDFKI FSIEACGILP GMTVKDAASN
LAEVCVIISV ISKGKIEYPP DRSYKIGAGD YVVFLAKNKK EVEEAIKG
