TVP18_YEAST
ID TVP18_YEAST Reviewed; 167 AA.
AC Q04767; D6VZP5;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Golgi apparatus membrane protein TVP18;
DE AltName: Full=TLG2 compartment vesicle protein of 18 kDa;
GN Name=TVP18; OrderedLocusNames=YMR071C; ORFNames=YM9916.10C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=16107716; DOI=10.1128/mcb.25.17.7696-7710.2005;
RA Inadome H., Noda Y., Adachi H., Yoda K.;
RT "Immunoisolation of the yeast Golgi subcompartments and characterization of
RT a novel membrane protein, Svp26, discovered in the Sed5-containing
RT compartments.";
RL Mol. Cell. Biol. 25:7696-7710(2005).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH TVP15 AND YIP4.
RX PubMed=17178117; DOI=10.1016/j.yexcr.2006.11.008;
RA Inadome H., Noda Y., Kamimura Y., Adachi H., Yoda K.;
RT "Tvp38, Tvp23, Tvp18 and Tvp15: novel membrane proteins in the Tlg2-
RT containing Golgi/endosome compartments of Saccharomyces cerevisiae.";
RL Exp. Cell Res. 313:688-697(2007).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-153, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-146 AND THR-153, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Golgi membrane protein involved in vesicular trafficking.
CC {ECO:0000269|PubMed:17178117}.
CC -!- SUBUNIT: Interacts with TVP15 and YIP4. {ECO:0000269|PubMed:17178117}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:16107716,
CC ECO:0000269|PubMed:17178117}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:16107716,
CC ECO:0000269|PubMed:17178117}.
CC -!- SIMILARITY: Belongs to the TVP18 family. {ECO:0000305}.
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DR EMBL; Z48952; CAA88796.1; -; Genomic_DNA.
DR EMBL; AY558402; AAS56728.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09969.1; -; Genomic_DNA.
DR PIR; S52831; S52831.
DR RefSeq; NP_013787.1; NM_001182569.1.
DR AlphaFoldDB; Q04767; -.
DR BioGRID; 35246; 77.
DR DIP; DIP-1731N; -.
DR IntAct; Q04767; 14.
DR MINT; Q04767; -.
DR STRING; 4932.YMR071C; -.
DR iPTMnet; Q04767; -.
DR SwissPalm; Q04767; -.
DR MaxQB; Q04767; -.
DR PaxDb; Q04767; -.
DR PRIDE; Q04767; -.
DR EnsemblFungi; YMR071C_mRNA; YMR071C; YMR071C.
DR GeneID; 855093; -.
DR KEGG; sce:YMR071C; -.
DR SGD; S000004675; TVP18.
DR VEuPathDB; FungiDB:YMR071C; -.
DR eggNOG; ENOG502S3AC; Eukaryota.
DR HOGENOM; CLU_118698_1_0_1; -.
DR InParanoid; Q04767; -.
DR OMA; ETNYMRA; -.
DR BioCyc; YEAST:G3O-32773-MON; -.
DR PRO; PR:Q04767; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04767; protein.
DR GO; GO:0030173; C:integral component of Golgi membrane; IDA:SGD.
DR GO; GO:0016192; P:vesicle-mediated transport; IGI:SGD.
DR InterPro; IPR019365; TVP18/Ca-channel_flower.
DR PANTHER; PTHR13314; PTHR13314; 1.
DR Pfam; PF10233; Cg6151-P; 1.
DR SMART; SM01077; Cg6151-P; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Golgi apparatus; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..167
FT /note="Golgi apparatus membrane protein TVP18"
FT /id="PRO_0000203281"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 146
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 153
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:19779198"
FT CARBOHYD 22
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 167 AA; 18693 MW; 46698DC0400D3097 CRC64;
MALSLGQFIN VGGMVKDLKS FNFSVYGRWF GYINIILCIA LGIANLFHVS GVIAFGIISI
IQGLVILFIE IPFLLKICPL SDNFIEFIKR FETNGWRCLF YLAMAIIQYI SIAVMATSLI
VVAVGLTISS ISYAVAYTKH QEFQNTNIIK NPTDDDFPHE AVVREML
