TVP23_ARATH
ID TVP23_ARATH Reviewed; 186 AA.
AC Q8LEK2; A0A178WGW7; Q0WRM9; Q9ZPZ2;
DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Golgi apparatus membrane protein-like protein ECHIDNA {ECO:0000303|PubMed:21512130};
GN Name=ECH {ECO:0000303|PubMed:21512130};
GN OrderedLocusNames=At1g09330 {ECO:0000312|Araport:AT1G09330};
GN ORFNames=T31J12.5 {ECO:0000312|EMBL:AAD18099.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21512130; DOI=10.1073/pnas.1018371108;
RA Gendre D., Oh J., Boutte Y., Best J.G., Samuels L., Nilsson R., Uemura T.,
RA Marchant A., Bennett M.J., Grebe M., Bhalerao R.P.;
RT "Conserved Arabidopsis ECHIDNA protein mediates trans-Golgi-network
RT trafficking and cell elongation.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:8048-8053(2011).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBUNIT, SUBCELLULAR LOCATION, AND
RP INTERACTION WITH YIP4A AND YIP4B.
RC STRAIN=cv. Columbia;
RX PubMed=23832588; DOI=10.1105/tpc.113.112482;
RA Gendre D., McFarlane H.E., Johnson E., Mouille G., Sjoedin A., Oh J.,
RA Levesque-Tremblay G., Watanabe Y., Samuels L., Bhalerao R.P.;
RT "Trans-Golgi network localized ECHIDNA/Ypt interacting protein complex is
RT required for the secretion of cell wall polysaccharides in Arabidopsis.";
RL Plant Cell 25:2633-2646(2013).
CC -!- FUNCTION: Mediates trans-Golgi-network trafficking and cell elongation
CC (PubMed:21512130, PubMed:23832588). Required for keeping the
CC appropriate balance between secretory trafficking and vacuolar
CC targeting of a subset of proteins (PubMed:21512130). The ECH/YIP4
CC complex is involved in the modulation of the trans-Golgi network (TGN)-
CC mediated trafficking of some proteins and cell wall components (e.g.
CC pectin and hemicellulose) to the cell wall in dark-grown hypocotyls and
CC in secretory cells of the seed coat (PubMed:23832588).
CC {ECO:0000269|PubMed:21512130, ECO:0000269|PubMed:23832588}.
CC -!- SUBUNIT: Component of a trans-Golgi network (TGN)-localized ECH/YIP4
CC complex made of ECH, YIP4A and YIP4B (PubMed:23832588). Interacts
CC directly with YIP4A and YIP4B (PubMed:23832588).
CC {ECO:0000269|PubMed:23832588}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000269|PubMed:23832588}; Multi-pass membrane protein
CC {ECO:0000255}. Early endosome membrane; Multi-pass membrane protein.
CC -!- DISRUPTION PHENOTYPE: Severe size reduction of all organs
CC (PubMed:21512130, PubMed:23832588). Abnormal cell wall composition
CC (PubMed:23832588). {ECO:0000269|PubMed:21512130,
CC ECO:0000269|PubMed:23832588}.
CC -!- SIMILARITY: Belongs to the TVP23 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD18099.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006416; AAD18099.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28430.1; -; Genomic_DNA.
DR EMBL; BT004772; AAO44038.1; -; mRNA.
DR EMBL; AK228273; BAF00220.1; -; mRNA.
DR EMBL; AY085379; AAM62608.1; -; mRNA.
DR PIR; D86226; D86226.
DR RefSeq; NP_563842.1; NM_100803.4.
DR AlphaFoldDB; Q8LEK2; -.
DR BioGRID; 22695; 9.
DR IntAct; Q8LEK2; 4.
DR STRING; 3702.AT1G09330.1; -.
DR iPTMnet; Q8LEK2; -.
DR PaxDb; Q8LEK2; -.
DR PRIDE; Q8LEK2; -.
DR ProteomicsDB; 234661; -.
DR EnsemblPlants; AT1G09330.1; AT1G09330.1; AT1G09330.
DR GeneID; 837454; -.
DR Gramene; AT1G09330.1; AT1G09330.1; AT1G09330.
DR KEGG; ath:AT1G09330; -.
DR Araport; AT1G09330; -.
DR TAIR; locus:2203023; AT1G09330.
DR eggNOG; KOG3195; Eukaryota.
DR HOGENOM; CLU_074845_2_0_1; -.
DR InParanoid; Q8LEK2; -.
DR OMA; DGNNHWK; -.
DR OrthoDB; 1356321at2759; -.
DR PhylomeDB; Q8LEK2; -.
DR PRO; PR:Q8LEK2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8LEK2; baseline and differential.
DR Genevisible; Q8LEK2; AT.
DR GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0005576; C:extracellular region; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0005802; C:trans-Golgi network; IDA:UniProtKB.
DR GO; GO:0032588; C:trans-Golgi network membrane; IDA:UniProtKB.
DR GO; GO:0007030; P:Golgi organization; IMP:UniProtKB.
DR GO; GO:0099402; P:plant organ development; IMP:UniProtKB.
DR GO; GO:0009306; P:protein secretion; IMP:UniProtKB.
DR GO; GO:0009826; P:unidimensional cell growth; IMP:UniProtKB.
DR GO; GO:0007034; P:vacuolar transport; IMP:TAIR.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR InterPro; IPR008564; TVP23-like.
DR PANTHER; PTHR13019; PTHR13019; 1.
DR Pfam; PF05832; DUF846; 1.
PE 1: Evidence at protein level;
KW Acetylation; Endosome; Golgi apparatus; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..186
FT /note="Golgi apparatus membrane protein-like protein
FT ECHIDNA"
FT /id="PRO_0000212835"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 186 AA; 21238 MW; 3C3FA8CD29B16D37 CRC64;
MDPNNQIQAP VENYANPRTC LFHVLFKGAA LAFYILSALF FNSFVIIFVV TVLLAALDFW
VVKNVSGRIL VGLRWWNEIN DLGESVWKFE SLDQESLARM NKKDSWLFWW TLYLAAAAWF
ILGVFSLIRF QADYLLVVGV CLSLNVANII GFTKCKKDAK KQFQQFASQT IASRFQSTVQ
SAFTLV
