ID   TWF_DROPS               Reviewed;         345 AA.
AC   Q298X4;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2009, sequence version 2.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Twinfilin;
GN   Name=twf; ORFNames=GA16421;
OS   Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=46245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MV2-25 / Tucson 14011-0121.94;
RX   PubMed=15632085; DOI=10.1101/gr.3059305;
RA   Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA   Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA   Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA   Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA   Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA   Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA   Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA   Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA   Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA   Weinstock G.M., Gibbs R.A.;
RT   "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT   gene, and cis-element evolution.";
RL   Genome Res. 15:1-18(2005).
CC   -!- FUNCTION: Actin-binding protein involved in motile and morphological
CC       processes. Inhibits actin polymerization, likely by sequestering G-
CC       actin (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with G-actin; ADP-actin form. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}. Cytoplasm,
CC       cell cortex {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the actin-binding proteins ADF family. Twinfilin
CC       subfamily. {ECO:0000305}.
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DR   EMBL; CM000070; EAL27831.2; -; Genomic_DNA.
DR   RefSeq; XP_001358688.2; XM_001358651.4.
DR   AlphaFoldDB; Q298X4; -.
DR   SMR; Q298X4; -.
DR   STRING; 7237.FBpp0282086; -.
DR   EnsemblMetazoa; FBtr0283648; FBpp0282086; FBgn0076436.
DR   GeneID; 4801632; -.
DR   KEGG; dpo:Dpse_GA16421; -.
DR   eggNOG; KOG1747; Eukaryota.
DR   HOGENOM; CLU_031995_0_1_1; -.
DR   InParanoid; Q298X4; -.
DR   OMA; VKKDWER; -.
DR   Proteomes; UP000001819; Chromosome 2.
DR   Bgee; FBgn0076436; Expressed in male reproductive system and 3 other tissues.
DR   GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:0098793; C:presynapse; IEA:GOC.
DR   GO; GO:0003779; F:actin binding; ISS:UniProtKB.
DR   GO; GO:0030042; P:actin filament depolymerization; IEA:EnsemblMetazoa.
DR   GO; GO:0007298; P:border follicle cell migration; IEA:EnsemblMetazoa.
DR   GO; GO:0008407; P:chaeta morphogenesis; IEA:EnsemblMetazoa.
DR   GO; GO:0016319; P:mushroom body development; IEA:EnsemblMetazoa.
DR   GO; GO:0030837; P:negative regulation of actin filament polymerization; IEA:EnsemblMetazoa.
DR   GO; GO:0007274; P:neuromuscular synaptic transmission; IEA:EnsemblMetazoa.
DR   GO; GO:0030833; P:regulation of actin filament polymerization; ISS:UniProtKB.
DR   GO; GO:0010591; P:regulation of lamellipodium assembly; IEA:EnsemblMetazoa.
DR   GO; GO:0048488; P:synaptic vesicle endocytosis; IEA:EnsemblMetazoa.
DR   Gene3D; 3.40.20.10; -; 2.
DR   InterPro; IPR002108; ADF-H.
DR   InterPro; IPR029006; ADF-H/Gelsolin-like_dom_sf.
DR   InterPro; IPR028458; Twinfilin.
DR   PANTHER; PTHR13759; PTHR13759; 1.
DR   Pfam; PF00241; Cofilin_ADF; 2.
DR   SMART; SM00102; ADF; 2.
DR   PROSITE; PS51263; ADF_H; 2.
PE   3: Inferred from homology;
KW   Actin-binding; Cytoplasm; Cytoskeleton; Reference proteome; Repeat.
FT   CHAIN           1..345
FT                   /note="Twinfilin"
FT                   /id="PRO_0000308813"
FT   DOMAIN          4..139
FT                   /note="ADF-H 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00599"
FT   DOMAIN          177..312
FT                   /note="ADF-H 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00599"
FT   REGION          320..345
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   345 AA;  39246 MW;  D710A1D47893C1A1 CRC64;
     MSHQTGIRAN EQLAKVLGKA KNGKLRVVKV SIENEQLSCS ATADVKKDWE RDYDKLLGPL
     LEETVPCYIL YRLDAKIPLG HSWLLISWIP DTASIRQKMV YASTKATLKT EFGSAYITEE
     LHATTLEETT LEGYRKHKRD FAAPAPLTTR EEELKELRKT EVHTEISTNT RHQTLGGISC
     PLTDATVAAV QDLVRGNYDY LQFRIDLEEE RIHVSHAAQV ELSALPKQVP EDHARYHLFL
     FRHTHEGDYQ ESYVFVYSMP GYTCSVRERM MYSSCKAPFL EQLAALGVDV VKKLEIDNGN
     ELTEAYLLDE LHPKKILHRP AFAKPKGPPN RGAKRLTRPS NEDQV