TWIST_BRABE
ID TWIST_BRABE Reviewed; 196 AA.
AC O96642;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=Twist-related protein;
DE AltName: Full=BBtwist;
GN Name=TWIST;
OS Branchiostoma belcheri (Amphioxus).
OC Eukaryota; Metazoa; Chordata; Cephalochordata; Leptocardii; Amphioxiformes;
OC Branchiostomidae; Branchiostoma.
OX NCBI_TaxID=7741 {ECO:0000312|EMBL:AAD10038.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Subsp. tsingtauense; TISSUE=Neurula;
RX PubMed=9520323; DOI=10.1006/dbio.1997.8834;
RA Yasui K., Zhang S.-C., Uemura M., Aizawa S., Ueki T.;
RT "Expression of a twist-related gene, Bbtwist, during the development of a
RT lancelet species and its relation to cephalochordate anterior structures.";
RL Dev. Biol. 195:49-59(1998).
CC -!- FUNCTION: Probable transcription factor with a role in initial mesoderm
CC differentiation. {ECO:0000303|PubMed:9520323}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Homodimer (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: In late gastrula embryos, expressed in the
CC presumptive first pair of somites and the middorsal wall of the
CC primitive gut. Expression is then up-regulated in the lateral wall of
CC the somites and the notochord. At the late neurula stage, also
CC expressed in the anterior wall of the primitive gut and in the
CC evaginating lateral diverticula. In the earliest larval stage,
CC expression is restricted to both ends of the body. In 36 hour larvae,
CC only faint expression is detected in the notochordal and paraxial
CC mesoderm of the caudal region. {ECO:0000269|PubMed:9520323}.
CC -!- DEVELOPMENTAL STAGE: Expressed in late gastrulae stage G6 to larval
CC stage L2. {ECO:0000269|PubMed:9520323}.
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DR EMBL; AF097914; AAD10038.1; -; mRNA.
DR AlphaFoldDB; O96642; -.
DR SMR; O96642; -.
DR Proteomes; UP000515135; Unplaced.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007498; P:mesoderm development; NAS:UniProtKB.
DR GO; GO:0045596; P:negative regulation of cell differentiation; NAS:UniProtKB.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR015789; Twist-related.
DR PANTHER; PTHR23349:SF100; PTHR23349:SF100; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..196
FT /note="Twist-related protein"
FT /id="PRO_0000127492"
FT DOMAIN 95..146
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 35..59
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 196 AA; 21625 MW; 717A279AA5EA0425 CRC64;
MAADVVTYTT LETFSHEDLV KPDPDASPLA DHSSSGPEAE EDPSRSKRYE RKRRYSKSSA
SSDDGSTGGG SVGGGKSGKN RKKTSKAESF EDLQNQRVLA NVRERQRTQS LNEAFSSLRK
IIPTLPSDKL SKIQTLKLAA RYIDFLYQVL RSDDTDTKMA SSCSYVAHER LSYAFSVSRQ
ELYFGLGAPS SGPNHF