ID   TWIST_PODCA             Reviewed;         199 AA.
AC   Q9GNV2;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Twist-related protein {ECO:0000250|UniProtKB:Q11094};
GN   Name=TWIST {ECO:0000303|PubMed:11112336};
OS   Podocoryna carnea (Hydrozoan).
OC   Eukaryota; Metazoa; Cnidaria; Hydrozoa; Hydroidolina; Anthoathecata;
OC   Filifera; Hydractiniidae; Podocoryna.
OX   NCBI_TaxID=6096;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:CAC12667.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP   STAGE.
RX   PubMed=11112336; DOI=10.1006/dbio.2000.9956;
RA   Spring J., Yanze N., Middel A.M., Stierwald M., Groger H., Schmid V.;
RT   "The mesoderm specification factor twist in the life cycle of jellyfish.";
RL   Dev. Biol. 228:363-375(2000).
CC   -!- FUNCTION: Probable transcription factor, which may be responsible for
CC       the formation of myoepithelial cells in early muscle development in
CC       larva and the formation of non-muscle tissues in later bud stages and
CC       mesoderm-like structures in the medusa. {ECO:0000269|PubMed:11112336}.
CC   -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC       protein. Homodimer (By similarity). {ECO:0000250|UniProtKB:Q11094}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q15672,
CC       ECO:0000255|PROSITE-ProRule:PRU00981}.
CC   -!- TISSUE SPECIFICITY: Expression is seen at the point of medusa formation
CC       in the ectodermal and endodermal bud tissues, and in the entocodon
CC       which gives rise to all smooth and striated muscle cells. After the
CC       subumbrellar plate differentiates from the endoderm, strong expression
CC       is detected until the medusa detaches from the gonzoid. Expression is
CC       observed in the distal part of the medusa but diminishes in entocodon-
CC       derived muscles as the tissues differentiate, with expression
CC       disappearing completely after stage 8. In later stages expression is
CC       seen in the distal and proximal parts of the bud and depending on state
CC       of maturity, in the developing gonadal tissue.
CC       {ECO:0000269|PubMed:11112336}.
CC   -!- DEVELOPMENTAL STAGE: Detected in the embryo and early planula larva
CC       stage. It disappears when larvae transform to the polyp stage after 2-3
CC       days. Strong expression is seen in the gonozoid and in all stages of
CC       medusa bud development. {ECO:0000269|PubMed:11112336}.
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DR   EMBL; AJ276245; CAC12667.1; -; mRNA.
DR   AlphaFoldDB; Q9GNV2; -.
DR   SMR; Q9GNV2; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   InterPro; IPR015789; Twist-related.
DR   PANTHER; PTHR23349:SF50; PTHR23349:SF50; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Differentiation; DNA-binding; Nucleus;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..199
FT                   /note="Twist-related protein"
FT                   /id="PRO_0000389516"
FT   DOMAIN          51..102
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          1..43
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        15..38
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   199 AA;  23358 MW;  C7FF8C93D29C0463 CRC64;
     MQEHQLSRVT SGNKKKYQSF DDESRDEKRM KCDSTDKLES NSNSKNIYQK THRVIANIRE
     RQRTQALNQS FSTLRKIIPT LPSDKLSKIQ TLRLAAMYID FLRHVIRRGE INMDSSDETF
     FSAQERLSYA FSVWRMEGDF YSRDKTAHYF TEQELNLCEY NFHSSFGNRL FYKAGIEAVN
     SADSDDITCI LNTFLEGRI