TWST2_RAT
ID TWST2_RAT Reviewed; 160 AA.
AC P97831; Q60981;
DT 11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Twist-related protein 2;
GN Name=Twist2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Wistar {ECO:0000305}; TISSUE=Aorta {ECO:0000305};
RA Kemp P., Grainger D., Metcalfe J.;
RT "Expression of helix-loop-helix proteins in vascular smooth muscle.";
RL Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Binds to the E-box consensus sequence 5'-CANNTG-3' as a
CC heterodimer and inhibits transcriptional activation by MYOD1, MYOG,
CC MEF2A and MEF2C. Also represses expression of pro-inflammatory
CC cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage
CC and energy metabolism (By similarity). Inhibits the premature or
CC ectopic differentiation of preosteoblast cells during osteogenesis,
CC possibly by changing the internal signal transduction response of
CC osteoblasts to external growth factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Forms a heterodimer with TCF3/E12. Also interacts with MEF2C
CC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC Cytoplasm {ECO:0000250}. Note=Mainly nuclear during embryonic
CC development. Cytoplasmic in adult tissues (By similarity).
CC {ECO:0000250}.
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DR EMBL; Y08139; CAA69333.1; -; mRNA.
DR RefSeq; NP_067723.1; NM_021691.2.
DR AlphaFoldDB; P97831; -.
DR SMR; P97831; -.
DR STRING; 10116.ENSRNOP00000027597; -.
DR PhosphoSitePlus; P97831; -.
DR PaxDb; P97831; -.
DR Ensembl; ENSRNOT00000027597; ENSRNOP00000027597; ENSRNOG00000020355.
DR GeneID; 59327; -.
DR KEGG; rno:59327; -.
DR CTD; 117581; -.
DR RGD; 621286; Twist2.
DR eggNOG; KOG4447; Eukaryota.
DR GeneTree; ENSGT00940000161996; -.
DR HOGENOM; CLU_112073_0_1_1; -.
DR InParanoid; P97831; -.
DR OMA; SNQSYEE; -.
DR OrthoDB; 1595261at2759; -.
DR PhylomeDB; P97831; -.
DR TreeFam; TF315153; -.
DR PRO; PR:P97831; -.
DR Proteomes; UP000002494; Chromosome 9.
DR Bgee; ENSRNOG00000020355; Expressed in esophagus and 17 other tissues.
DR Genevisible; P97831; RN.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005730; C:nucleolus; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; ISO:RGD.
DR GO; GO:0003682; F:chromatin binding; ISO:RGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:RGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0019904; F:protein domain specific binding; ISO:RGD.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0061303; P:cornea development in camera-type eye; ISO:RGD.
DR GO; GO:0032502; P:developmental process; IBA:GO_Central.
DR GO; GO:0048701; P:embryonic cranial skeleton morphogenesis; ISO:RGD.
DR GO; GO:0060325; P:face morphogenesis; ISO:RGD.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IDA:RGD.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:RGD.
DR GO; GO:0043392; P:negative regulation of DNA binding; ISO:RGD.
DR GO; GO:0044092; P:negative regulation of molecular function; ISO:RGD.
DR GO; GO:0045638; P:negative regulation of myeloid cell differentiation; ISO:RGD.
DR GO; GO:0045668; P:negative regulation of osteoblast differentiation; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0032720; P:negative regulation of tumor necrosis factor production; ISO:RGD.
DR GO; GO:0001649; P:osteoblast differentiation; ISO:RGD.
DR GO; GO:0030335; P:positive regulation of cell migration; ISO:RGD.
DR GO; GO:0010838; P:positive regulation of keratinocyte proliferation; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:RGD.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR015789; Twist-related.
DR PANTHER; PTHR23349:SF70; PTHR23349:SF70; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Developmental protein; Differentiation; DNA-binding; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..160
FT /note="Twist-related protein 2"
FT /id="PRO_0000127491"
FT DOMAIN 66..117
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 160 AA; 18124 MW; 8F44750916940C0A CRC64;
MEEGSSSPVS PVDSLGTSEE ELERQPKRFG RKRRYSKKSS EDGSPTPGKR GKKGSPSAQS
FEELQSQRIL ANVRERQRTQ SLNEAFAALR KIIPTLPSDK LSKIQTLKLA ARYIDFLYQV
LQSDEMDNKM TSCSYVAHER LSYAFSVWRM EGAWSMSASH
