TX101_HUMAN
ID TX101_HUMAN Reviewed; 249 AA.
AC Q9BY14; Q7L5R2; Q9BPY7;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Testis-expressed protein 101 {ECO:0000305};
DE AltName: Full=Cell surface receptor NYD-SP8;
DE AltName: Full=Scleroderma-associated autoantigen;
DE AltName: Full=Spermatogenesis-related gene protein;
DE Flags: Precursor;
GN Name=TEX101 {ECO:0000312|HGNC:HGNC:30722}; Synonyms=SGRG;
GN ORFNames=UNQ867/PRO1884;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=16516155; DOI=10.1016/j.bbrc.2006.01.175;
RA Teng X., Yang J., Xie Y., Ni Z., Hu R., Shi L., Lin Z., Hu L., Zhao G.,
RA Ding X., Kong X.;
RT "A novel spermatogenesis-specific uPAR gene expressed in human and mouse
RT testis.";
RL Biochem. Biophys. Res. Commun. 342:1223-1227(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RA Li J.M., Zhou Z.M., Sha J.H., Lin M., Wang L.R., Zhou Y.D., Zhu H.H.,
RA Zhu H.;
RT "NYD-SP8: a novel gene related to human testis development.";
RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RA Yang J., Xie Y., Ni Z., Wang Z., Zhong L., Lei H., Hu L.L., Shi L.,
RA Zhao G., Kong X.;
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Plays a role in fertilization by controlling binding of sperm
CC to zona pellucida and migration of spermatozoa into the oviduct (By
CC similarity). May play a role in signal transduction and promote protein
CC tyrosine phosphorylation (By similarity).
CC {ECO:0000250|UniProtKB:Q924B5, ECO:0000250|UniProtKB:Q9JMI7}.
CC -!- SUBUNIT: Interacts with VAMP3. Interacts with LY6K. Interacts with
CC DPEP3; co-localized on the cell surface of spermatocytes, spermatids,
CC and testicular spermatozoa, co-localized only in cytoplasmic droplets
CC of caput and corpus epididymal sperm. Interacts with ADAM5.
CC {ECO:0000250|UniProtKB:Q9JMI7}.
CC -!- INTERACTION:
CC Q9BY14-2; Q9P2G9-2: KLHL8; NbExp=3; IntAct=EBI-12306161, EBI-11959635;
CC Q9BY14-2; Q9NRD5: PICK1; NbExp=3; IntAct=EBI-12306161, EBI-79165;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9JMI7};
CC Lipid-anchor, GPI-anchor {ECO:0000250|UniProtKB:Q9JMI7}. Membrane raft
CC {ECO:0000250|UniProtKB:Q9JMI7}. Cytoplasmic vesicle, secretory vesicle,
CC acrosome {ECO:0000250|UniProtKB:Q9JMI7}. Secreted
CC {ECO:0000250|UniProtKB:Q9JMI7}. Cytoplasmic vesicle
CC {ECO:0000250|UniProtKB:Q9JMI7}. Note=Located on plasma membrane of
CC spermatocytes, round and elongated spermatids, and testicular
CC spermatozoa. {ECO:0000250|UniProtKB:Q9JMI7}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9BY14-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9BY14-2; Sequence=VSP_020029;
CC -!- TISSUE SPECIFICITY: Detected in testis and spermatogonia. Not detected
CC in spermatocytes. Detected in blood leukocytes.
CC {ECO:0000269|PubMed:16516155}.
CC -!- PTM: N-glycosylated; by high mannose and/or biantennary complex and/or
CC certain types of hybrid oligosaccharides; possesses different
CC oligosaccharides chains according to its subcellular localization in
CC the testis. {ECO:0000250|UniProtKB:Q9JMI7}.
CC -!- PTM: Sheds from membrane raft by ACE and released from the cell surface
CC of epididymal sperm while it passes through the caput epididymis
CC leading to disappearance of TEX101 on spermatozoa; is essential to
CC produce fertile spermatozoa. {ECO:0000250|UniProtKB:Q9JMI7}.
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DR EMBL; AF241268; AAK28327.1; -; mRNA.
DR EMBL; AY014285; AAK27310.1; -; mRNA.
DR EMBL; AF353396; AAK38662.1; -; mRNA.
DR EMBL; AY359077; AAQ89436.1; -; mRNA.
DR EMBL; BC001861; AAH01861.2; -; mRNA.
DR CCDS; CCDS12619.1; -. [Q9BY14-2]
DR CCDS; CCDS59393.1; -. [Q9BY14-1]
DR RefSeq; NP_001123483.1; NM_001130011.1. [Q9BY14-1]
DR RefSeq; NP_113639.4; NM_031451.4. [Q9BY14-2]
DR PDB; 7BPR; X-ray; 1.95 A; A/B=26-222.
DR PDBsum; 7BPR; -.
DR AlphaFoldDB; Q9BY14; -.
DR SMR; Q9BY14; -.
DR BioGRID; 123703; 255.
DR IntAct; Q9BY14; 269.
DR STRING; 9606.ENSP00000472308; -.
DR GlyGen; Q9BY14; 2 sites.
DR iPTMnet; Q9BY14; -.
DR PhosphoSitePlus; Q9BY14; -.
DR BioMuta; TEX101; -.
DR DMDM; 110816451; -.
DR MassIVE; Q9BY14; -.
DR PaxDb; Q9BY14; -.
DR PeptideAtlas; Q9BY14; -.
DR PRIDE; Q9BY14; -.
DR ProteomicsDB; 79563; -. [Q9BY14-1]
DR ProteomicsDB; 79564; -. [Q9BY14-2]
DR Antibodypedia; 31041; 40 antibodies from 17 providers.
DR DNASU; 83639; -.
DR Ensembl; ENST00000598265.2; ENSP00000472769.1; ENSG00000131126.19. [Q9BY14-1]
DR Ensembl; ENST00000602198.5; ENSP00000472308.1; ENSG00000131126.19. [Q9BY14-2]
DR GeneID; 83639; -.
DR KEGG; hsa:83639; -.
DR MANE-Select; ENST00000598265.2; ENSP00000472769.1; NM_001130011.3; NP_001123483.1.
DR UCSC; uc010xwo.3; human. [Q9BY14-1]
DR CTD; 83639; -.
DR DisGeNET; 83639; -.
DR GeneCards; TEX101; -.
DR HGNC; HGNC:30722; TEX101.
DR HPA; ENSG00000131126; Tissue enriched (testis).
DR MIM; 612665; gene.
DR neXtProt; NX_Q9BY14; -.
DR OpenTargets; ENSG00000131126; -.
DR PharmGKB; PA134938764; -.
DR VEuPathDB; HostDB:ENSG00000131126; -.
DR eggNOG; ENOG502T90B; Eukaryota.
DR GeneTree; ENSGT00530000063351; -.
DR HOGENOM; CLU_077973_0_0_1; -.
DR InParanoid; Q9BY14; -.
DR OMA; QETVLMI; -.
DR PhylomeDB; Q9BY14; -.
DR TreeFam; TF337286; -.
DR PathwayCommons; Q9BY14; -.
DR Reactome; R-HSA-163125; Post-translational modification: synthesis of GPI-anchored proteins.
DR SignaLink; Q9BY14; -.
DR BioGRID-ORCS; 83639; 16 hits in 1064 CRISPR screens.
DR ChiTaRS; TEX101; human.
DR GenomeRNAi; 83639; -.
DR Pharos; Q9BY14; Tbio.
DR PRO; PR:Q9BY14; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q9BY14; protein.
DR Bgee; ENSG00000131126; Expressed in right testis and 120 other tissues.
DR ExpressionAtlas; Q9BY14; baseline and differential.
DR Genevisible; Q9BY14; HS.
DR GO; GO:0002080; C:acrosomal membrane; IEA:Ensembl.
DR GO; GO:0001669; C:acrosomal vesicle; ISS:UniProtKB.
DR GO; GO:0046658; C:anchored component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0005576; C:extracellular region; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0044853; C:plasma membrane raft; IBA:GO_Central.
DR GO; GO:0007339; P:binding of sperm to zona pellucida; ISS:UniProtKB.
DR GO; GO:0009566; P:fertilization; ISS:UniProtKB.
DR GO; GO:0030317; P:flagellated sperm motility; ISS:UniProtKB.
DR GO; GO:1901317; P:regulation of flagellated sperm motility; ISS:UniProtKB.
DR InterPro; IPR016054; LY6_UPA_recep-like.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR Pfam; PF00021; UPAR_LY6; 1.
DR SUPFAM; SSF57302; SSF57302; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell membrane; Cytoplasmic vesicle;
KW Glycoprotein; GPI-anchor; Lipoprotein; Membrane; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000250"
FT CHAIN 26..222
FT /note="Testis-expressed protein 101"
FT /id="PRO_0000247621"
FT PROPEP 223..249
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000247622"
FT DOMAIN 140..211
FT /note="UPAR/Ly6"
FT LIPID 222
FT /note="GPI-anchor amidated asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 159
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1
FT /note="M -> MGARQIQTSSSQTSPEEAM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16516155"
FT /id="VSP_020029"
FT VARIANT 99
FT /note="G -> V (in dbSNP:rs35033974)"
FT /id="VAR_059883"
FT CONFLICT 157
FT /note="C -> Y (in Ref. 1; AAK28327)"
FT /evidence="ECO:0000305"
FT STRAND 27..29
FT /evidence="ECO:0007829|PDB:7BPR"
FT STRAND 31..39
FT /evidence="ECO:0007829|PDB:7BPR"
FT HELIX 41..43
FT /evidence="ECO:0007829|PDB:7BPR"
FT STRAND 51..53
FT /evidence="ECO:0007829|PDB:7BPR"
FT STRAND 59..69
FT /evidence="ECO:0007829|PDB:7BPR"
FT STRAND 72..84
FT /evidence="ECO:0007829|PDB:7BPR"
FT STRAND 88..95
FT /evidence="ECO:0007829|PDB:7BPR"
FT STRAND 97..99
FT /evidence="ECO:0007829|PDB:7BPR"
FT STRAND 101..109
FT /evidence="ECO:0007829|PDB:7BPR"
FT HELIX 124..126
FT /evidence="ECO:0007829|PDB:7BPR"
FT STRAND 139..141
FT /evidence="ECO:0007829|PDB:7BPR"
FT STRAND 143..148
FT /evidence="ECO:0007829|PDB:7BPR"
FT STRAND 154..156
FT /evidence="ECO:0007829|PDB:7BPR"
FT STRAND 163..173
FT /evidence="ECO:0007829|PDB:7BPR"
FT STRAND 176..186
FT /evidence="ECO:0007829|PDB:7BPR"
FT TURN 193..196
FT /evidence="ECO:0007829|PDB:7BPR"
FT STRAND 203..208
FT /evidence="ECO:0007829|PDB:7BPR"
FT VARIANT Q9BY14-2:4
FT /note="R -> M (in dbSNP:rs17849541)"
FT /evidence="ECO:0000305"
FT /id="VAR_082931"
SQ SEQUENCE 249 AA; 26667 MW; CEDC82D7C4F04821 CRC64;
MGTPRIQHLL ILLVLGASLL TSGLELYCQK GLSMTVEADP ANMFNWTTEE VETCDKGALC
QETILIIKAG TETAILATKG CIPEGEEAIT IVQHSSPPGL IVTSYSNYCE DSFCNDKDSL
SQFWEFSETT ASTVSTTLHC PTCVALGTCF SAPSLPCPNG TTRCYQGKLE ITGGGIESSV
EVKGCTAMIG CRLMSGILAV GPMFVREACP HQLLTQPRKT ENGATCLPIP VWGLQLLLPL
LLPSFIHFS
