ID   TX10A_MANRB             Reviewed;          82 AA.
AC   A0A6G9KJK9;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   12-AUG-2020, sequence version 1.
DT   25-MAY-2022, entry version 5.
DE   RecName: Full=U10-myrmicitoxin-Mri1a {ECO:0000303|PubMed:32182430};
DE            Short=U10-MYRTX-Mri1a {ECO:0000303|PubMed:32182430};
DE   Flags: Precursor;
OS   Manica rubida (European giant red ant).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC   Formicidae; Myrmicinae; Manica.
OX   NCBI_TaxID=219785 {ECO:0000312|EMBL:QIQ51446.1};
RN   [1] {ECO:0000312|EMBL:QIQ51446.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, TOXIC DOSE, MASS SPECTROMETRY, AND AMIDATION AT ILE-81.
RC   TISSUE=Venom gland {ECO:0000312|EMBL:QIQ51446.1};
RX   PubMed=32182430; DOI=10.1021/acs.jproteome.0c00048;
RA   Touchard A., Aili S.R., Tene N., Barasse V., Klopp C., Dejean A.,
RA   Kini R.M., Mrinalini X., Coquet L., Jouenne T., Lefranc B., Leprince J.,
RA   Escoubas P., Nicholson G.M., Treilhou M., Bonnafe E.;
RT   "Venom Peptide Repertoire of the European Myrmicine Ant Manica rubida:
RT   Identification of Insecticidal Toxins.";
RL   J. Proteome Res. 19:1800-1811(2020).
CC   -!- FUNCTION: Induces paralysis after injection into blowflies (L.caesar)
CC       (PubMed:32182430). In most cases is not lethal 24 hours after
CC       injection, but paralysis is irreversible (PubMed:32182430). May have
CC       antimicrobial properties, like most ant linear peptides (By
CC       similarity). {ECO:0000250|UniProtKB:Q07932,
CC       ECO:0000269|PubMed:32182430}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:32182430}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:32182430}.
CC   -!- MASS SPECTROMETRY: Mass=2850.7; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:32182430};
CC   -!- TOXIC DOSE: PD(50) is 12.12 +-1.5 nmol/g in blowfly (L.caesar) (at 1
CC       hour post-injection). {ECO:0000269|PubMed:32182430}.
CC   -!- TOXIC DOSE: PD(50) is 20.45 +-7.0 nmol/g in blowfly (L.caesar) (at 24
CC       hours post-injection). {ECO:0000269|PubMed:32182430}.
CC   -!- SIMILARITY: Belongs to the formicidae venom precursor-01 superfamily.
CC       {ECO:0000305}.
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DR   EMBL; MN765036; QIQ51446.1; -; mRNA.
DR   AlphaFoldDB; A0A6G9KJK9; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0035738; P:envenomation resulting in modulation of process in another organism; IDA:UniProtKB.
DR   GO; GO:0044616; P:modulation of relaxation of muscle in another organism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amidation; Antimicrobial; Secreted; Signal; Toxin.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   PROPEP          27..52
FT                   /evidence="ECO:0000305|PubMed:32182430"
FT                   /id="PRO_0000453059"
FT   PEPTIDE         53..81
FT                   /note="U10-myrmicitoxin-Mri1a"
FT                   /evidence="ECO:0000269|PubMed:32182430"
FT                   /id="PRO_0000453060"
FT   MOD_RES         81
FT                   /note="Isoleucine amide"
FT                   /evidence="ECO:0000305|PubMed:32182430"
SQ   SEQUENCE   82 AA;  8265 MW;  BFE34B28BB9A719D CRC64;
     MRLSYISLTI AIIFVMAIVH APETEAKAYP EADAVAEAIA VGEADAVGVA DPGFKSMLAK
     AALKILKAVA PAAAAAIADK IG