ID   TX10B_MANRB             Reviewed;          81 AA.
AC   A0A6G9KJV6;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   12-AUG-2020, sequence version 1.
DT   25-MAY-2022, entry version 5.
DE   RecName: Full=U10-myrmicitoxin-Mri1b {ECO:0000303|PubMed:32182430};
DE            Short=U10-MYRTX-Mri1b {ECO:0000303|PubMed:32182430};
DE   Flags: Precursor;
OS   Manica rubida (European giant red ant).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC   Formicidae; Myrmicinae; Manica.
OX   NCBI_TaxID=219785 {ECO:0000312|EMBL:QIQ51444.1};
RN   [1] {ECO:0000312|EMBL:QIQ51444.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, MASS SPECTROMETRY, TOXIC DOSE, AND AMIDATION AT VAL-80.
RC   TISSUE=Venom gland {ECO:0000312|EMBL:QIQ51444.1};
RX   PubMed=32182430; DOI=10.1021/acs.jproteome.0c00048;
RA   Touchard A., Aili S.R., Tene N., Barasse V., Klopp C., Dejean A.,
RA   Kini R.M., Mrinalini X., Coquet L., Jouenne T., Lefranc B., Leprince J.,
RA   Escoubas P., Nicholson G.M., Treilhou M., Bonnafe E.;
RT   "Venom Peptide Repertoire of the European Myrmicine Ant Manica rubida:
RT   Identification of Insecticidal Toxins.";
RL   J. Proteome Res. 19:1800-1811(2020).
CC   -!- FUNCTION: Induces paralysis after injection into blowflies (L.caesar),
CC       and then death within 24 hours (PubMed:32182430). May have
CC       antimicrobial properties, like most ant linear peptides (By
CC       similarity). {ECO:0000250|UniProtKB:Q07932,
CC       ECO:0000269|PubMed:32182430}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:32182430}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:32182430}.
CC   -!- MASS SPECTROMETRY: Mass=2987.8; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:32182430};
CC   -!- TOXIC DOSE: LD(50) is 97.97 +-7.3 nmol/g in blowfly (L.caesar) (at 24
CC       hours post-injection). {ECO:0000269|PubMed:32182430}.
CC   -!- TOXIC DOSE: PD(50) is 58.39 +-7.9 nmol/g in blowfly (L.caesar) (at 1
CC       hour post-injection). {ECO:0000269|PubMed:32182430}.
CC   -!- TOXIC DOSE: PD(50) is 67.37 +-14 nmol/g in blowfly (L.caesar) (at 24
CC       hours post-injection). {ECO:0000269|PubMed:32182430}.
CC   -!- SIMILARITY: Belongs to the formicidae venom precursor-01 superfamily.
CC       {ECO:0000305}.
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DR   EMBL; MN765034; QIQ51444.1; -; mRNA.
DR   AlphaFoldDB; A0A6G9KJV6; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0035738; P:envenomation resulting in modulation of process in another organism; IDA:UniProtKB.
DR   GO; GO:0044616; P:modulation of relaxation of muscle in another organism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amidation; Antimicrobial; Secreted; Signal; Toxin.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   PROPEP          27..52
FT                   /evidence="ECO:0000305|PubMed:32182430"
FT                   /id="PRO_0000453061"
FT   PEPTIDE         53..80
FT                   /note="U10-myrmicitoxin-Mri1b"
FT                   /evidence="ECO:0000269|PubMed:32182430"
FT                   /id="PRO_0000453062"
FT   MOD_RES         80
FT                   /note="Valine amide"
FT                   /evidence="ECO:0000305|PubMed:32182430"
SQ   SEQUENCE   81 AA;  8402 MW;  440AE5E7873D8128 CRC64;
     MRLSYISLTL AIIFVMAIVH APETEAKAYP EADAVAEAIA VGEADAVGVA DPGIKDALAK
     IWKILKAEVP TVAAAIENKV G