ID   TX12A_MANRB             Reviewed;          37 AA.
AC   A0A6G9KHD3;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   12-AUG-2020, sequence version 1.
DT   25-MAY-2022, entry version 4.
DE   RecName: Full=U12-myrmicitoxin-Mri1a {ECO:0000303|PubMed:32182430};
DE            Short=U12-MYRTX-Mri1a {ECO:0000303|PubMed:32182430};
DE   AltName: Full=U-MYRTX-MANr1 {ECO:0000303|PubMed:31557881};
DE   Flags: Precursor;
OS   Manica rubida (European giant red ant).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC   Formicidae; Myrmicinae; Manica.
OX   NCBI_TaxID=219785 {ECO:0000312|EMBL:QIQ51447.1};
RN   [1] {ECO:0000312|EMBL:QIQ51447.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-35, FUNCTION,
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, AND AMIDATION
RP   AT VAL-35.
RC   TISSUE=Venom gland {ECO:0000312|EMBL:QIQ51447.1};
RX   PubMed=32182430; DOI=10.1021/acs.jproteome.0c00048;
RA   Touchard A., Aili S.R., Tene N., Barasse V., Klopp C., Dejean A.,
RA   Kini R.M., Mrinalini X., Coquet L., Jouenne T., Lefranc B., Leprince J.,
RA   Escoubas P., Nicholson G.M., Treilhou M., Bonnafe E.;
RT   "Venom Peptide Repertoire of the European Myrmicine Ant Manica rubida:
RT   Identification of Insecticidal Toxins.";
RL   J. Proteome Res. 19:1800-1811(2020).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 26-35, FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, MASS SPECTROMETRY, AND AMIDATION AT VAL-35.
RC   TISSUE=Venom gland {ECO:0000303|PubMed:31557881};
RX   PubMed=31557881; DOI=10.3390/toxins11100562;
RA   Heep J., Skaljac M., Grotmann J., Kessel T., Seip M., Schmidtberg H.,
RA   Vilcinskas A.;
RT   "Identification and Functional Characterization of a Novel Insecticidal
RT   Decapeptide from the Myrmicine Ant Manica rubida.";
RL   Toxins 11:0-0(2019).
CC   -!- FUNCTION: Toxin that induces mild paralysis, and reduces survival and
CC       reproduction when injected into aphids (A.pisum) (PubMed:31557881). May
CC       affect various processes in the aphid, including wound healing and
CC       hemolymph coagulation (PubMed:31557881). It does not increase the
CC       sensitivity of the aphids to the chemical insecticides imidacloprid,
CC       methomyl and Spirotetramat (PubMed:31557881). Has no insecticidal
CC       activity when injected into blowfly (L.caesar) (PubMed:32182430). Does
CC       not display any antibacterial or antifungal activity (PubMed:31557881).
CC       {ECO:0000269|PubMed:31557881, ECO:0000269|PubMed:32182430}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:31557881,
CC       ECO:0000269|PubMed:32182430}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:31557881, ECO:0000269|PubMed:32182430}.
CC   -!- MASS SPECTROMETRY: Mass=1110.7; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:31557881, ECO:0000269|PubMed:32182430};
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DR   EMBL; MN765037; QIQ51447.1; -; mRNA.
DR   AlphaFoldDB; A0A6G9KHD3; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0044468; P:envenomation resulting in modulation of blood coagulation in another organism; IDA:UniProtKB.
DR   GO; GO:0044616; P:modulation of relaxation of muscle in another organism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Secreted; Signal; Toxin.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   PROPEP          24..25
FT                   /evidence="ECO:0000305|PubMed:31557881,
FT                   ECO:0000305|PubMed:32182430"
FT                   /id="PRO_0000453051"
FT   PEPTIDE         26..35
FT                   /note="U12-myrmicitoxin-Mri1a"
FT                   /evidence="ECO:0000269|PubMed:31557881,
FT                   ECO:0000269|PubMed:32182430"
FT                   /id="PRO_0000453052"
FT   MOD_RES         35
FT                   /note="Valine amide"
FT                   /evidence="ECO:0000269|PubMed:31557881,
FT                   ECO:0000269|PubMed:32182430"
SQ   SEQUENCE   37 AA;  3989 MW;  3D384DE675FDAAEB CRC64;
     MKTIELITIF AMITTLMVTV VAGDPIDPKV LESLVGK