ID   TX12A_ODOMO             Reviewed;          81 AA.
AC   A0A348G5V8;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   07-NOV-2018, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=U-poneritoxin(01)-Om2a {ECO:0000250|UniProtKB:A0A348G5W2};
DE            Short=U-PONTX(01)-Om2a {ECO:0000250|UniProtKB:A0A348G5W2};
DE   AltName: Full=Pilosulin-like peptide 2 {ECO:0000312|EMBL:BBF97831.1};
DE            Short=PLP2 {ECO:0000303|PubMed:29027956};
DE   AltName: Full=Poneratoxin {ECO:0000305};
DE   Flags: Precursor;
OS   Odontomachus monticola (Trap-jaw ant).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC   Formicidae; Ponerinae; Ponerini; Odontomachus.
OX   NCBI_TaxID=613454;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 48-66, MASS SPECTROMETRY,
RP   AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=29027956; DOI=10.3390/toxins9100323;
RA   Kazuma K., Masuko K., Konno K., Inagaki H.;
RT   "Combined venom gland transcriptomic and venom peptidomic analysis of the
RT   predatory ant Odontomachus monticola.";
RL   Toxins 9:323-323(2017).
RN   [2]
RP   FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SYNTHESIS OF PEPTIDE WITH
RP   UNKNOWN TERMINAL RESIDUES, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=30658410; DOI=10.3390/toxins11010050;
RA   Tani N., Kazuma K., Ohtsuka Y., Shigeri Y., Masuko K., Konno K.,
RA   Inagaki H.;
RT   "Mass spectrometry analysis and biological characterization of the
RT   predatory ant Odontomachus monticola venom and venom sac components.";
RL   Toxins 11:0-0(2019).
CC   -!- FUNCTION: Cationic amphipathic alpha-helical peptide with antimicrobial
CC       activities against E.coli (MIC=6.2 uM), S.aureus (MIC=6.2 uM), and
CC       S.cerevisiae (MIC=50 uM). Also shows histamine-releasing activity
CC       (30.1% at 10 uM) and a weak hemolytic activity (10.4% at 50 uM).
CC       {ECO:0000269|PubMed:30658410}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:29027956}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:29027956}.
CC   -!- PTM: Truncated sequences of this peptide have also been found in the
CC       venom. It is possible they have been cleaved in the venom.
CC       {ECO:0000305|PubMed:29027956, ECO:0000305|PubMed:30658410}.
CC   -!- MASS SPECTROMETRY: Mass=3361.756; Method=Electrospray;
CC       Note=Monoisotopic mass.; Evidence={ECO:0000269|PubMed:29027956};
CC   -!- SIMILARITY: Belongs to the formicidae venom precursor-01 superfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FX985495; BBF97831.1; -; mRNA.
DR   AlphaFoldDB; A0A348G5V8; -.
DR   SMR; A0A348G5V8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing; Hemolysis;
KW   Secreted; Signal; Toxin.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   PROPEP          26..47
FT                   /evidence="ECO:0000305|PubMed:29027956"
FT                   /id="PRO_0000447069"
FT   PEPTIDE         48..80
FT                   /note="U-poneritoxin(01)-Om2a"
FT                   /evidence="ECO:0000269|PubMed:30658410,
FT                   ECO:0000305|PubMed:29027956"
FT                   /id="PRO_5016905946"
SQ   SEQUENCE   81 AA;  8379 MW;  A671F81BD02BB427 CRC64;
     MKPSGITFAF LVVFMMAIMY NSVQAAAIAD ADADAEAKAF ADAFAEAGWG SIFKTVGKMI
     AKAAVKAAPE AISAMASQNE K