ID   TX132_APHSP             Reviewed;          42 AA.
AC   P0CI04;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 1.
DT   25-MAY-2022, entry version 24.
DE   RecName: Full=Omega-theraphotoxin-Asp3a;
DE            Short=Omega-TRTX-Asp3a;
DE   AltName: Full=Peptide 7-13.2 {ECO:0000303|Ref.1};
OS   Aphonopelma sp. (American tarantula).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Aphonopelma; unclassified Aphonopelma.
OX   NCBI_TaxID=29932;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Venom;
RA   Nason D.M., Phillips D., Saccomano N.A., Volkmann R.A.;
RT   "Calcium channel blocking polypeptides from Theraphosidae Aphonopelma.";
RL   Patent number EP0668872, 13-MAY-1998.
CC   -!- FUNCTION: Inhibits voltage-gated calcium channels (Cav) in rat
CC       cerebellar granule cells. {ECO:0000269|Ref.1}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|Ref.1}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knottin'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 14 (magi-1) family. 08 (Ltx-4)
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0CI04; -.
DR   SMR; P0CI04; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR012627; Toxin_22.
DR   Pfam; PF08092; Toxin_22; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated calcium channel impairing toxin.
FT   CHAIN           1..42
FT                   /note="Omega-theraphotoxin-Asp3a"
FT                   /id="PRO_0000401918"
FT   DISULFID        1..16
FT                   /evidence="ECO:0000250"
FT   DISULFID        8..21
FT                   /evidence="ECO:0000250"
FT   DISULFID        15..30
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   42 AA;  4851 MW;  909EFC583AB7A4FC CRC64;
     CLGENVPCDK DRPNCCSKYE CLEPTGYGRC YASYYSYKKK TL