ID   C5I1_RHIAP              Reviewed;          98 AA.
AC   A0A141SFN4; A0A158RFT5;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   08-JUN-2016, sequence version 1.
DT   03-AUG-2022, entry version 8.
DE   RecName: Full=Complement inhibitor RaCI1 {ECO:0000303|PubMed:27018802};
DE   AltName: Full=Rhipicephalus appendiculatus complement inhibitor 1 {ECO:0000312|EMBL:AML25523.1};
DE   Flags: Precursor;
OS   Rhipicephalus appendiculatus (Brown ear tick).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Rhipicephalinae;
OC   Rhipicephalus; Rhipicephalus.
OX   NCBI_TaxID=34631;
RN   [1] {ECO:0000312|EMBL:AML25523.1, ECO:0007744|PDB:5HCE}
RP   NUCLEOTIDE SEQUENCE [MRNA], X-RAY CRYSTALLOGRAPHY (3.12 ANGSTROMS) OF 21-98
RP   IN COMPLEX WITH HUMAN COMPLEMENT C5 AND THE TICK COMPLEMENT INHBIBITOR
RP   OMCI, AND FUNCTION.
RC   TISSUE=Salivary gland;
RX   PubMed=27018802; DOI=10.1038/nsmb.3196;
RA   Jore M.M., Johnson S., Sheppard D., Barber N.M., Li Y.I., Nunn M.A.,
RA   Elmlund H., Lea S.M.;
RT   "Structural basis for therapeutic inhibition of complement C5.";
RL   Nat. Struct. Mol. Biol. 23:378-386(2016).
RN   [2] {ECO:0007744|PDB:6RQJ}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS) OF 21-98 IN COMPLEX WITH
RP   HUMAN COMPLEMENT C5 AND THE TICK COMPLEMENT INHBIBITORS OMCI AND CIRPT1,
RP   AND DISULFIDE BONDS.
RX   PubMed=31871188; DOI=10.1073/pnas.1909973116;
RA   Reichhardt M.P., Johnson S., Tang T., Morgan T., Tebeka N., Popitsch N.,
RA   Deme J.C., Jore M.M., Lea S.M.;
RT   "An inhibitor of complement C5 provides structural insights into
RT   activation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 117:362-370(2020).
CC   -!- FUNCTION: Complement inhibitor (PubMed:27018802). Prevents complement-
CC       mediated C5 activation by binding to C5 (PubMed:27018802). Binds C5 at
CC       a different binding site than the other tick complement inbibitors OmCI
CC       and CirpT1, and the drug eculizumab (By similarity). Inhibits the
CC       complement in human and guinea pig but not in other species tested
CC       (rabbit, rat, mouse, and pig) (PubMed:27018802).
CC       {ECO:0000250|UniProtKB:A0A146B485, ECO:0000269|PubMed:27018802}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:27018802}.
CC   -!- TISSUE SPECIFICITY: Expressed by salivary glands.
CC       {ECO:0000305|PubMed:27018802}.
CC   -!- SIMILARITY: Belongs to the RaCI family. {ECO:0000305}.
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DR   EMBL; KU533794; AML25523.1; -; mRNA.
DR   PDB; 5HCE; X-ray; 3.12 A; D=21-98.
DR   PDB; 6RQJ; EM; 3.50 A; D=21-98.
DR   PDBsum; 5HCE; -.
DR   PDBsum; 6RQJ; -.
DR   SMR; A0A141SFN4; -.
PE   1: Evidence at protein level;
KW   3D-structure; Complement system impairing toxin; Disulfide bond; Secreted;
KW   Signal; Toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..98
FT                   /note="Complement inhibitor RaCI1"
FT                   /id="PRO_5007492727"
FT   DISULFID        33..57
FT                   /evidence="ECO:0000269|PubMed:27018802,
FT                   ECO:0007744|PDB:6RQJ"
FT   DISULFID        38..59
FT                   /evidence="ECO:0000269|PubMed:27018802,
FT                   ECO:0007744|PDB:6RQJ"
FT   DISULFID        53..74
FT                   /evidence="ECO:0000269|PubMed:27018802,
FT                   ECO:0007744|PDB:6RQJ"
SQ   SEQUENCE   98 AA;  10487 MW;  48607A8DB6D422C0 CRC64;
     MNAMLVLFIA SALFISEHNT EEVKTTPIPN HQCVNATCER KLDALGNAVI TKCPQGCLCV
     VRGASNIVPA NGTCFQLATT KPPMAPGDNK DNKEEESN