ID   C5I2_RHIMP              Reviewed;          98 AA.
AC   A0A158RFT4; A0A182DWE4;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 2.
DT   03-AUG-2022, entry version 13.
DE   RecName: Full=Complement inhibitor RaCI2 {ECO:0000303|PubMed:27018802};
DE   Flags: Precursor;
OS   Rhipicephalus microplus (Cattle tick) (Boophilus microplus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Rhipicephalinae;
OC   Rhipicephalus; Boophilus.
OX   NCBI_TaxID=6941;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Salivary gland;
RX   PubMed=20298599; DOI=10.1186/1471-2164-11-186;
RA   Zivkovic Z., Esteves E., Almazan C., Daffre S., Nijhof A.M., Kocan K.M.,
RA   Jongejan F., de la Fuente J.;
RT   "Differential expression of genes in salivary glands of male Rhipicephalus
RT   (Boophilus)microplus in response to infection with Anaplasma marginale.";
RL   BMC Genomics 11:186-186(2010).
RN   [2] {ECO:0000312|PDB:5HCD, ECO:0000312|PDB:5IEC}
RP   X-RAY CRYSTALLOGRAPHY (2.98 ANGSTROMS) OF 22-98 IN COMPLEX WITH HUMAN
RP   COMPLEMENT C5 AND THE TICK COMPLEMENT INHBIBITOR OMCI, STRUCTURE BY NMR OF
RP   22-88, FUNCTION, DISULFIDE BONDS, AND MUTAGENESIS OF 22-GLU--VAL-31 AND
RP   87-THR--GLU-98.
RC   TISSUE=Salivary gland;
RX   PubMed=27018802; DOI=10.1038/nsmb.3196;
RA   Jore M.M., Johnson S., Sheppard D., Barber N.M., Li Y.I., Nunn M.A.,
RA   Elmlund H., Lea S.M.;
RT   "Structural basis for therapeutic inhibition of complement C5.";
RL   Nat. Struct. Mol. Biol. 23:378-386(2016).
CC   -!- FUNCTION: Complement inhibitor (PubMed:27018802). Prevents complement-
CC       mediated C5 activation by binding to C5 (PubMed:27018802). Binds C5 at
CC       a different binding site than the other tick complement inbibitors OmCI
CC       and CirpT1, and the drug eculizumab (By similarity).
CC       {ECO:0000250|UniProtKB:A0A146B485, ECO:0000269|PubMed:27018802}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:27018802}.
CC   -!- TISSUE SPECIFICITY: Expressed by salivary glands.
CC       {ECO:0000305|PubMed:27018802}.
CC   -!- SIMILARITY: Belongs to the RaCI family. {ECO:0000305}.
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DR   EMBL; GO496246; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GO496255; -; NOT_ANNOTATED_CDS; mRNA.
DR   PDB; 5HCD; X-ray; 2.98 A; D=22-98.
DR   PDB; 5IEC; NMR; -; A=22-88.
DR   PDBsum; 5HCD; -.
DR   PDBsum; 5IEC; -.
DR   BMRB; A0A158RFT4; -.
DR   SMR; A0A158RFT4; -.
PE   1: Evidence at protein level;
KW   3D-structure; Complement system impairing toxin; Disulfide bond; Secreted;
KW   Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..98
FT                   /note="Complement inhibitor RaCI2"
FT                   /id="PRO_0000456230"
FT   DISULFID        35..59
FT                   /evidence="ECO:0000269|PubMed:27018802,
FT                   ECO:0007744|PDB:5HCD, ECO:0007744|PDB:5IEC"
FT   DISULFID        40..61
FT                   /evidence="ECO:0000269|PubMed:27018802,
FT                   ECO:0007744|PDB:5HCD, ECO:0007744|PDB:5IEC"
FT   DISULFID        55..76
FT                   /evidence="ECO:0000269|PubMed:27018802,
FT                   ECO:0007744|PDB:5HCD, ECO:0007744|PDB:5IEC"
FT   MUTAGEN         22..31
FT                   /note="Missing: No change in activity."
FT                   /evidence="ECO:0000269|PubMed:27018802"
FT   MUTAGEN         87..98
FT                   /note="Missing: No change in activity."
FT                   /evidence="ECO:0000269|PubMed:27018802"
SQ   SEQUENCE   98 AA;  10633 MW;  14B6E2D09FD3AACF CRC64;
     MNAVTVLAFT AFALIVHDCY SEEANTTPIS VKDQCANVTC RRTVDNRGKR HIDGCPPGCL
     CVLKGPDSKD NLDGTCYLLA TTPKSTTTST EQSFNMEE