TX19B_MOUSE
ID TX19B_MOUSE Reviewed; 317 AA.
AC Q9D5S1;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Testis-expressed protein 19.2;
DE AltName: Full=Testis-expressed protein 19B;
GN Name=Tex19.2; Synonyms=Tex19b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=18096721; DOI=10.1634/stemcells.2007-0772;
RA Kuntz S., Kieffer E., Bianchetti L., Lamoureux N., Fuhrmann G., Viville S.;
RT "Tex19, a mammalian-specific protein with a restricted expression in
RT pluripotent stem cells and germ line.";
RL Stem Cells 26:734-744(2008).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP INTERACTION WITH UBR2.
RX PubMed=21103378; DOI=10.1371/journal.pone.0014017;
RA Yang F., Cheng Y., An J.Y., Kwon Y.T., Eckardt S., Leu N.A.,
RA McLaughlin K.J., Wang P.J.;
RT "The ubiquitin ligase Ubr2, a recognition E3 component of the N-end rule
RT pathway, stabilizes Tex19.1 during spermatogenesis.";
RL PLoS ONE 5:E14017-E14017(2010).
RN [6]
RP DEVELOPMENTAL STAGE.
RX PubMed=22447323; DOI=10.1262/jrd.11-047k;
RA Celebi C., van Montfoort A., Skory V., Kieffer E., Kuntz S., Mark M.,
RA Viville S.;
RT "Tex 19 paralogs exhibit a gonad and placenta-specific expression in the
RT mouse.";
RL J. Reprod. Dev. 58:360-365(2012).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, RNA-BINDING, AND
RP INTERACTION WITH DDX4; EDC4; MAEL; PIWIL1; PIWIL2; RANBP9 AND TDRD6.
RX PubMed=28254886; DOI=10.1242/jcs.188763;
RA Tarabay Y., Achour M., Teletin M., Ye T., Teissandier A., Mark M.,
RA Bourc'his D., Viville S.;
RT "Tex19 paralogs are new members of the piRNA pathway controlling
RT retrotransposon suppression.";
RL J. Cell Sci. 130:1463-1474(2017).
CC -!- FUNCTION: May be required during spermatogenesis, probably by
CC participating in the repression of retrotransposable elements and
CC prevent their mobilization (Probable). With its paralog, Tex19.1,
CC collaborates with the Piwi-interacting RNA (piRNA) pathway, which
CC mediates the repression of transposable elements during meiosis by
CC forming complexes composed of piRNAs and Piwi proteins. Interacts with
CC Piwi proteins and directly binds piRNAs, a class of 24 to 30 nucleotide
CC RNAs that are generated by a Dicer-independent mechanism and are
CC primarily derived from transposons and other repeated sequence elements
CC (PubMed:28254886). {ECO:0000269|PubMed:28254886,
CC ECO:0000305|PubMed:28254886}.
CC -!- SUBUNIT: Interacts with UBR2 (PubMed:21103378). Interacts with piRNA-
CC associated proteins DDX4, EDC4, MAEL, PIWIL1, PIWIL2, RANBP9 and TDRD6
CC (Probable). {ECO:0000269|PubMed:21103378, ECO:0000305|PubMed:28254886}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q99MV2}.
CC Note=Was initially reported to localize in the nucleus.
CC {ECO:0000269|PubMed:18096721}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in somatic cells of male
CC gonad lineage. {ECO:0000269|PubMed:18096721,
CC ECO:0000269|PubMed:28254886}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the ectoderm and then in primordial
CC germ cells (PGCs). Expressed in testis from embryos (13.5 dpc) to
CC adulthood in gonocytes and spermatocytes.
CC {ECO:0000269|PubMed:22447323}.
CC -!- DISRUPTION PHENOTYPE: Males deficient for both for Tex19.1 and Tex19.2
CC have impaired spermatogenesis, small testes and are infertile. They
CC show vacuolization and seminiferous epithelium degeneration as early as
CC P16. They have defects in meiotic chromosome synapsis, persistence of
CC DNA double-strand breaks during meiosis, lack of post-meiotic germ cell
CC and up-regulation of MMERVK10C retrotransposon expression. Number of
CC females double knockout for Tex19.1 and Tex19.2 surviving 2 weeks or
CC more is reduced compared to males. Females display normal fertility.
CC Surviving mutants do not present gross somatic abnormalities.
CC {ECO:0000269|PubMed:28254886}.
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DR EMBL; AK014985; BAB29655.1; -; mRNA.
DR EMBL; AL662887; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS25767.1; -.
DR RefSeq; NP_081898.1; NM_027622.3.
DR AlphaFoldDB; Q9D5S1; -.
DR STRING; 10090.ENSMUSP00000037027; -.
DR PaxDb; Q9D5S1; -.
DR PRIDE; Q9D5S1; -.
DR ProteomicsDB; 298069; -.
DR DNASU; 70956; -.
DR Ensembl; ENSMUST00000039146; ENSMUSP00000037027; ENSMUSG00000039337.
DR GeneID; 70956; -.
DR KEGG; mmu:70956; -.
DR UCSC; uc007mvh.2; mouse.
DR CTD; 70956; -.
DR MGI; MGI:1918206; Tex19.2.
DR VEuPathDB; HostDB:ENSMUSG00000039337; -.
DR eggNOG; ENOG502T8GZ; Eukaryota.
DR GeneTree; ENSGT00390000014279; -.
DR HOGENOM; CLU_855143_0_0_1; -.
DR InParanoid; Q9D5S1; -.
DR OMA; QQDLHRW; -.
DR OrthoDB; 805493at2759; -.
DR PhylomeDB; Q9D5S1; -.
DR TreeFam; TF338441; -.
DR BioGRID-ORCS; 70956; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q9D5S1; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q9D5S1; protein.
DR Bgee; ENSMUSG00000039337; Expressed in spermatocyte and 32 other tissues.
DR ExpressionAtlas; Q9D5S1; baseline and differential.
DR Genevisible; Q9D5S1; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0034584; F:piRNA binding; IDA:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0008584; P:male gonad development; IBA:GO_Central.
DR GO; GO:0007140; P:male meiotic nuclear division; IMP:UniProtKB.
DR GO; GO:0010529; P:negative regulation of transposition; ISO:MGI.
DR GO; GO:0001890; P:placenta development; IBA:GO_Central.
DR GO; GO:0007283; P:spermatogenesis; IMP:UniProtKB.
DR InterPro; IPR029093; TEX19.
DR PANTHER; PTHR31387; PTHR31387; 1.
DR Pfam; PF15553; TEX19; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Differentiation; Meiosis; Reference proteome; RNA-binding;
KW Spermatogenesis.
FT CHAIN 1..317
FT /note="Testis-expressed protein 19.2"
FT /id="PRO_0000325964"
FT REGION 64..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 101..145
FT /note="Important for interaction with piRNA"
FT /evidence="ECO:0000269|PubMed:28254886"
SQ SEQUENCE 317 AA; 35235 MW; 700FEDB9B80A7F3C CRC64;
MCPPVSVRHG AKGMSCLYGA WLYHLVHGEQ MKICFACFKA AFLVVKNMLE MGDWEEGVWD
AEPMELSEAS SEPEEWPGLS GGEGQGHLPH GISVSAGSGA QGPQPVPTEL GPQEAVPLDL
GPEDAEWTQA LPWRFDGLSP CSHWLIPPLS WWEIFNVSPS PGQPVLLELS PTWPMDPLEA
EAWLVGLKFV FLLGGFDAIC YMLSMTPCWA VRTRVQRWQV LLDPGDVRVA QLQNAPEQQD
LHRWKLSVLE SSELGMELVP ADCSLQKGGF KVHSYLPWHN STPESWSREP GERLLVVEVV
SLRELPCFRS PSPDPHN
