ID   TX1A_APHSP              Reviewed;          39 AA.
AC   P0DL81;
DT   22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2017, sequence version 1.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=Omega-theraphotoxin-Asp1a {ECO:0000305};
DE            Short=Omega-TRTX-Asp1a {ECO:0000305};
DE   AltName: Full=Peptide 6-6 {ECO:0000303|Ref.1};
OS   Aphonopelma sp. (American tarantula).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Aphonopelma; unclassified Aphonopelma.
OX   NCBI_TaxID=29932;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RA   Nason D.M.I., Douglas P., Saccomano N.A., Volkmann R.A.;
RT   "Calcium channel blocking polypeptides from theraphosidae aphonopelma.";
RL   Patent number WO1994010196, 11-MAY-1994.
CC   -!- FUNCTION: Toxin that inhibits voltage-gated calcium channels in rat
CC       cerebellar granule cells (IC(50)<200 nM) (Ref.1). Is lethal to
CC       cockroaches (By similarity). {ECO:0000250|UniProtKB:P61509,
CC       ECO:0000269|Ref.1}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|Ref.1}.
CC   -!- MASS SPECTROMETRY: Mass=4382.16; Mass_error=0.54; Method=Electrospray;
CC       Evidence={ECO:0000269|Ref.1};
CC   -!- SIMILARITY: Belongs to the neurotoxin 12 (Hwtx-2) family. 06 (TXP1)
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0DL81; -.
DR   SMR; P0DL81; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR012625; Toxin_20.
DR   Pfam; PF08089; Toxin_20; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated calcium channel impairing toxin.
FT   CHAIN           1..39
FT                   /note="Omega-theraphotoxin-Asp1a"
FT                   /evidence="ECO:0000269|Ref.1"
FT                   /id="PRO_0000442234"
FT   DISULFID        4..25
FT                   /evidence="ECO:0000250|UniProtKB:P0DL80"
FT   DISULFID        8..31
FT                   /evidence="ECO:0000250|UniProtKB:P0DL80"
FT   DISULFID        17..36
FT                   /evidence="ECO:0000250|UniProtKB:P0DL80"
SQ   SEQUENCE   39 AA;  4389 MW;  B6B29D1E3F97AD25 CRC64;
     LFECVLSCDI KKNGKPCKPK GEKKCSGGWR CKINFCLKV