ID   TX1A_PHOKE              Reviewed;          78 AA.
AC   P84062;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   19-JAN-2010, sequence version 2.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=U5-ctenitoxin-Pk1a;
DE            Short=U5-CNTX-Pk1a;
DE   AltName: Full=Neurotoxin PKTx15C1;
DE   AltName: Full=Neurotoxin PKTx1A;
OS   Phoneutria keyserlingi (Brazilian wandering spider) (Ctenus keyserlingii).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Lycosoidea; Ctenidae; Phoneutria.
OX   NCBI_TaxID=272754;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom {ECO:0000269|PubMed:16278100};
RX   PubMed=16278100; DOI=10.1016/j.cbpc.2005.09.010;
RA   Richardson M., Pimenta A.M., Bemquerer M.P., Santoro M.M., Beirao P.S.,
RA   Lima M.E., Figueiredo S.G., Bloch C. Jr., Vasconcelos E.A., Campos F.A.,
RA   Gomes P.C., Cordeiro M.N.;
RT   "Comparison of the partial proteomes of the venoms of Brazilian spiders of
RT   the genus Phoneutria.";
RL   Comp. Biochem. Physiol. 142:173-187(2006).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, AND VARIANT GLY-15.
RC   TISSUE=Venom {ECO:0000269|Ref.2};
RA   Richardson M., Pimenta A.M.C., Bemquerer M.P., Santoro M.M.,
RA   Figueiredo S.G., Cordeiro M.N.;
RT   "Neurotoxin PKTx1A from venom of spider Phoneutria keyserlingi has strong
RT   sequence identity with PNTx1 from Phoneutria nigriventer.";
RL   Submitted (JUN-2004) to UniProtKB.
CC   -!- FUNCTION: Lethal neurotoxin. Causes spastic paralysis and death in mice
CC       in 4-6 min after intracerebroventricular injection at dose levels of
CC       1.5 ug per mouse. {ECO:0000269|Ref.2}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16278100}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:16278100}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=8769.66; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:16278100};
CC   -!- SIMILARITY: Belongs to the neurotoxin 04 (omega-agtx) family. 02 (Tx1)
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P84062; -.
DR   ArachnoServer; AS000219; U5-ctenitoxin-Pk1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR013605; Toxin_34.
DR   Pfam; PF08396; Toxin_34; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Neurotoxin; Secreted;
KW   Toxin.
FT   CHAIN           1..78
FT                   /note="U5-ctenitoxin-Pk1a"
FT                   /id="PRO_0000087624"
FT   DISULFID        6..23
FT                   /evidence="ECO:0000305"
FT   DISULFID        13..29
FT                   /evidence="ECO:0000305"
FT   DISULFID        20..52
FT                   /evidence="ECO:0000255"
FT   DISULFID        22..40
FT                   /evidence="ECO:0000305"
FT   DISULFID        31..38
FT                   /evidence="ECO:0000305"
FT   DISULFID        58..73
FT                   /evidence="ECO:0000255"
FT   DISULFID        69..77
FT                   /evidence="ECO:0000255"
FT   VARIANT         15
FT                   /note="D -> G"
FT                   /evidence="ECO:0000269|Ref.2"
SQ   SEQUENCE   78 AA;  8745 MW;  90DD394F8C26D6C5 CRC64;
     AELKSCFPVG HECDDDASNC NCCGDDVYCA CGWGRWNCKC KVADQSYAYG ICKDKVNCPN
     RHLWPAKECK MPCRRNCG