TX1A_TLIAL
ID TX1A_TLIAL Reviewed; 99 AA.
AC P0DUC0;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 02-DEC-2020, sequence version 1.
DT 25-MAY-2022, entry version 4.
DE RecName: Full=U1-theraphotoxin-Tal1a {ECO:0000305};
DE Short=U1-TRTX-Tal1a {ECO:0000305};
DE AltName: Full=Brachyin {ECO:0000303|PubMed:25329070};
DE Flags: Precursor;
OS Tliltocatl albopilosus (Curlyhair tarantula) (Brachypelma albopilosum).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Mygalomorphae; Theraphosidae; Brachypelma.
OX NCBI_TaxID=351119;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 58-98, FUNCTION, AMIDATION
RP AT SER-98, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND TOXIC DOSE.
RC TISSUE=Venom;
RX PubMed=25329070; DOI=10.1371/journal.pone.0110221;
RA Zhong Y., Song B., Mo G., Yuan M., Li H., Wang P., Yuan M., Lu Q.;
RT "A novel neurotoxin from venom of the spider, Brachypelma albopilosum.";
RL PLoS ONE 9:e110221-e110221(2014).
CC -!- FUNCTION: Insecticidal toxin that shows strong lethal effects on
CC American cockroaches (P.americana) and common mealbeetle (T.molitor)
CC (PubMed:25329070). Possibly acts by blocking ion channel currents
CC (Probable). Also shows significant analgesic effects in mice models of
CC pain including abdominal writhing induced by acetic acid and formalin-
CC induced paw licking tests (PubMed:25329070). In addition, it exerts
CC marked inhibition on tumor cell proliferation (PubMed:25329070).
CC {ECO:0000269|PubMed:25329070, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:25329070}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:25329070}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin. {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=4901.7; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:25329070};
CC -!- TOXIC DOSE: LD(50) is 5 ug/kg in adult cockroaches.
CC {ECO:0000269|PubMed:25329070}.
CC -!- TOXIC DOSE: LD(50) is 7.6 ug/kg in mealbeetles.
CC {ECO:0000269|PubMed:25329070}.
CC -!- SIMILARITY: Belongs to the neurotoxin 14 (magi-1) family. 08 (Ltx-4)
CC subfamily. {ECO:0000305}.
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DR AlphaFoldDB; P0DUC0; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR012627; Toxin_22.
DR Pfam; PF08092; Toxin_22; 1.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Disulfide bond; Ion channel impairing toxin; Knottin; Neurotoxin; Secreted;
KW Signal; Toxin.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..57
FT /evidence="ECO:0000305|PubMed:25329070"
FT /id="PRO_0000451469"
FT CHAIN 58..98
FT /note="U1-theraphotoxin-Tal1a"
FT /evidence="ECO:0000269|PubMed:25329070"
FT /id="PRO_0000451470"
FT MOD_RES 98
FT /note="Serine amide"
FT /evidence="ECO:0000269|PubMed:25329070"
FT DISULFID 58..73
FT /evidence="ECO:0000305"
FT DISULFID 65..78
FT /evidence="ECO:0000305"
FT DISULFID 72..93
FT /evidence="ECO:0000305"
SQ SEQUENCE 99 AA; 11315 MW; 65CBAC5B33123103 CRC64;
MNTIQVIIFA VVLVLTVTVG QADEDSAETS LLRKLKEAEA SLFGQHLEES QHSREKRCLG
ENVPCDKDRP NCCSRYECLE PTGYGWWYAS YYCYKKRSG
