ID   TX1A_TRICU              Reviewed;          60 AA.
AC   C0HL38;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2017, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Insect toxin mu-NPTX-Nc1a {ECO:0000303|PubMed:28497266};
DE   AltName: Full=Mu/kappa-nephilitoxin-Nc1a {ECO:0000305};
DE            Short=Mu/kappa-NPTX-Nc1a {ECO:0000305};
DE   Flags: Precursor;
OS   Trichonephila clavata (Joro spider) (Nephila clavata).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Araneoidea; Nephilidae; Trichonephila.
OX   NCBI_TaxID=2740835;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-60, FUNCTION,
RP   SUBCELLULAR LOCATION, MASS SPECTROMETRY, PRESENCE OF DISULFIDE BONDS, AND
RP   TOXIC DOSE.
RC   TISSUE=Venom;
RX   PubMed=28497266; DOI=10.1007/s00726-017-2425-2;
RA   Jin L., Fang M., Chen M., Zhou C., Ombati R., Hakim M.A., Mo G., Lai R.,
RA   Yan X., Wang Y., Yang S.;
RT   "An insecticidal toxin from Nephila clavata spider venom.";
RL   Amino Acids 49:1237-1245(2017).
CC   -!- FUNCTION: Insect-specific toxin. Blocks voltage-gated potassium and
CC       sodium channels. {ECO:0000269|PubMed:28497266}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28497266}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:28497266}.
CC   -!- PTM: Contains 4 disulfide bonds. {ECO:0000269|PubMed:28497266}.
CC   -!- MASS SPECTROMETRY: Mass=4234.9; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:28497266};
CC   -!- TOXIC DOSE: LD(50) is 573 ng/g in P.americana.
CC       {ECO:0000269|PubMed:28497266}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; C0HL38; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Potassium channel impairing toxin; Secreted; Signal; Toxin;
KW   Voltage-gated potassium channel impairing toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|PubMed:28497266"
FT   CHAIN           20..60
FT                   /note="Insect toxin mu-NPTX-Nc1a"
FT                   /evidence="ECO:0000269|PubMed:28497266"
FT                   /id="PRO_0000442018"
SQ   SEQUENCE   60 AA;  6209 MW;  FE99BACA0E2E7F4B CRC64;
     MIYQVVLLLL VSPAPVSAAG CNPDCTGIQC GWPRCPGGQN PVMDKCVSCC PFCPPKSAQG