TX1B_MONBA
ID TX1B_MONBA Reviewed; 38 AA.
AC P0DUC2;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 02-DEC-2020, sequence version 1.
DT 25-MAY-2022, entry version 6.
DE RecName: Full=Mu/omega-theraphotoxin-Mb1b {ECO:0000305|PubMed:28475112};
DE Short=Mu/omega-TRTX-Mb1b {ECO:0000303|PubMed:28475112};
OS Monocentropus balfouri (Socotra Island blue baboon tarantula).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Mygalomorphae; Theraphosidae; Monocentropus.
OX NCBI_TaxID=2053173;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, AMIDATION AT SER-38, MASS SPECTROMETRY,
RP SUBCELLULAR LOCATION, RECOMBINANT EXPRESSION, TOXIC DOSE, AND BIOASSAY.
RC TISSUE=Venom;
RX PubMed=28475112; DOI=10.3390/toxins9050155;
RA Smith J.J., Herzig V., Ikonomopoulou M.P., Dziemborowicz S., Bosmans F.,
RA Nicholson G.M., King G.F.;
RT "Insect-active toxins with promiscuous pharmacology from the african
RT theraphosid spider Monocentropus balfouri.";
RL Toxins 9:0-0(2017).
CC -!- FUNCTION: Paralytic toxin on insects that inhibits voltage-gated sodium
CC (Nav) and calcium (Cav) channels in P.americana (American cockroach)
CC dorsal unpaired median (DUM) neurons, and also inhibits the B.germanica
CC (German cockroach) Nav channel (BgNaV1) (PubMed:28475112). May act as a
CC gating-modifier toxin on Nav and as a pore blocker on Cav
CC (PubMed:28475112). In vivo, reversibly paralyzes both L.cuprina
CC (Australian sheep blowfly) and M.domestica (housefly), but does not
CC affect larvae of H.armigera (cotton bollworms) (PubMed:28475112).
CC {ECO:0000269|PubMed:28475112}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28475112}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:28475112}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin.
CC {ECO:0000250|UniProtKB:P0DM12}.
CC -!- MASS SPECTROMETRY: Mass=4147.00; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:28475112};
CC -!- TOXIC DOSE: PD(50) is 5.6+-0.2 nmol/g when intra-thoracically injected
CC into L.cuprina blowflies (at 30 minutes post-injection).
CC {ECO:0000269|PubMed:28475112}.
CC -!- TOXIC DOSE: PD(50) is 6.0+-0.2 nmol/g when intra-thoracically injected
CC into L.cuprina blowflies (at 1 hour post-injection).
CC {ECO:0000269|PubMed:28475112}.
CC -!- TOXIC DOSE: PD(50) is 7.7+-0.5 nmol/g when intra-thoracically injected
CC into L.cuprina blowflies (at 2 hours post-injection).
CC {ECO:0000269|PubMed:28475112}.
CC -!- MISCELLANEOUS: Possibly exists in two forms, due to cis-trans
CC isomerization of Pro-5 or Pro-22. {ECO:0000269|PubMed:28475112}.
CC -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family. 28 (Jztx-11)
CC subfamily. {ECO:0000305}.
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DR AlphaFoldDB; P0DUC2; -.
DR SMR; P0DUC2; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR011696; Huwentoxin-1.
DR Pfam; PF07740; Toxin_12; 1.
PE 1: Evidence at protein level;
KW Amidation; Calcium channel impairing toxin; Direct protein sequencing;
KW Disulfide bond; Ion channel impairing toxin; Knottin; Neurotoxin; Secreted;
KW Toxin; Voltage-gated calcium channel impairing toxin;
KW Voltage-gated sodium channel impairing toxin.
FT CHAIN 1..38
FT /note="Mu/omega-theraphotoxin-Mb1b"
FT /evidence="ECO:0000269|PubMed:28475112"
FT /id="PRO_0000451472"
FT MOD_RES 38
FT /note="Serine amide"
FT /evidence="ECO:0000269|PubMed:28475112"
FT DISULFID 7..21
FT /evidence="ECO:0000250|UniProtKB:P0DM12"
FT DISULFID 14..26
FT /evidence="ECO:0000250|UniProtKB:P0DM12"
FT DISULFID 20..33
FT /evidence="ECO:0000250|UniProtKB:P0DM12"
SQ SEQUENCE 38 AA; 4156 MW; 9DA490388BDD258D CRC64;
GVDKPGCRYM FGGCVQDDDC CPHLGCKRKG LYCAWDAS