ID   TX1F_APHSP              Reviewed;          39 AA.
AC   P61509; P18928;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2004, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Omega-theraphotoxin-Asp1f {ECO:0000305};
DE            Short=Omega-TRTX-Asp1f {ECO:0000305};
DE   AltName: Full=Eurypelma spider toxin {ECO:0000303|PubMed:2742756};
DE            Short=ESTx {ECO:0000303|PubMed:2742756};
OS   Aphonopelma sp. (American tarantula).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Aphonopelma; unclassified Aphonopelma.
OX   NCBI_TaxID=29932;
RN   [1]
RP   PROTEIN SEQUENCE, BIOASSAY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=2742756; DOI=10.1515/bchm3.1989.370.1.485;
RA   Savel-Niemann A.;
RT   "Tarantula (Eurypelma californicum) venom, a multicomponent system.";
RL   Biol. Chem. Hoppe-Seyler 370:485-498(1989).
CC   -!- FUNCTION: Insecticidal toxin that is lethal to cockroaches
CC       (PubMed:2742756). Inhibits voltage-gated calcium channels (Cav) in rat
CC       cerebellar granule cells (By similarity).
CC       {ECO:0000250|UniProtKB:P0DL81, ECO:0000269|PubMed:2742756}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:2742756}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:2742756}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 12 (Hwtx-2) family. 06 (TXP1)
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P61509; -.
DR   SMR; P61509; -.
DR   ArachnoServer; AS000222; omega-theraphotoxin-Asp1f.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR012625; Toxin_20.
DR   Pfam; PF08089; Toxin_20; 1.
DR   PROSITE; PS60022; HWTX_2; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated calcium channel impairing toxin.
FT   PEPTIDE         1..39
FT                   /note="Omega-theraphotoxin-Asp1f"
FT                   /evidence="ECO:0000269|PubMed:2742756"
FT                   /id="PRO_0000044980"
FT   DISULFID        4..25
FT                   /evidence="ECO:0000250|UniProtKB:P0DL80"
FT   DISULFID        8..31
FT                   /evidence="ECO:0000250|UniProtKB:P0DL80"
FT   DISULFID        17..36
FT                   /evidence="ECO:0000250|UniProtKB:P0DL80"
SQ   SEQUENCE   39 AA;  4419 MW;  E572A54E6496EF22 CRC64;
     IFECVFSCDI EKEGKPCKPK GEKKCTGGWK CKIKLCLKI