ID   C5IT4_AMBAM             Reviewed;         108 AA.
AC   P0DQV3;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Complement inhibitor CirpT4 {ECO:0000303|PubMed:31871188};
DE   Flags: Precursor;
OS   Amblyomma americanum (Lone star tick).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae; Amblyomma.
OX   NCBI_TaxID=6943;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Salivary gland;
RX   PubMed=19320755; DOI=10.1111/j.1365-2583.2009.00863.x;
RA   Aljamali M.N., Hern L., Kupfer D., Downard S., So S., Roe B.A., Sauer J.R.,
RA   Essenberg R.C.;
RT   "Transcriptome analysis of the salivary glands of the female tick Amblyomma
RT   americanum (Acari: Ixodidae).";
RL   Insect Mol. Biol. 18:129-154(2009).
RN   [2]
RP   FUNCTION, AND RECOMBINANT EXPRESSION.
RC   TISSUE=Salivary gland;
RX   PubMed=31871188; DOI=10.1073/pnas.1909973116;
RA   Reichhardt M.P., Johnson S., Tang T., Morgan T., Tebeka N., Popitsch N.,
RA   Deme J.C., Jore M.M., Lea S.M.;
RT   "An inhibitor of complement C5 provides structural insights into
RT   activation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 117:362-370(2020).
CC   -!- FUNCTION: Complement inhibitor (PubMed:31871188). Prevents complement-
CC       mediated activation of C5 by sterically preventing direct binding of C5
CC       to its convertase (binding with domains MG4 and MG5) (PubMed:31871188)
CC       (By similarity). Binds C5 at a different binding site than the other
CC       tick complement inbibitors OmCI and RaCI3, and the drug eculizumab (By
CC       similarity). Inhibits the complement in human, rat and guinea pig, and
CC       also shows a reduced inhibition in rabbit and pig (By similarity).
CC       {ECO:0000250|UniProtKB:L7MB58, ECO:0000269|PubMed:31871188}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:31871188}.
CC   -!- TISSUE SPECIFICITY: Expressed by the salivary glands.
CC       {ECO:0000305|PubMed:31871188}.
CC   -!- SIMILARITY: Belongs to the CirpT family. {ECO:0000305}.
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DR   EMBL; CX766574; -; NOT_ANNOTATED_CDS; mRNA.
PE   3: Inferred from homology;
KW   Disulfide bond; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..108
FT                   /note="Complement inhibitor CirpT4"
FT                   /id="PRO_0000456235"
FT   DISULFID        40..64
FT                   /evidence="ECO:0000250|UniProtKB:L7MB58"
FT   DISULFID        59..98
FT                   /evidence="ECO:0000250|UniProtKB:L7MB58"
FT   DISULFID        76..99
FT                   /evidence="ECO:0000250|UniProtKB:L7MB58"
FT   DISULFID        85..104
FT                   /evidence="ECO:0000250|UniProtKB:L7MB58"
SQ   SEQUENCE   108 AA;  12003 MW;  9614EF95322A7BCD CRC64;
     MRAFVALFCT LVAFATVICD IQEHGHSYLT RNVTVENGAC IFERNTLPDG ETKALHDPCV
     IATCYAARRE VNATLCRNFG VDPGCRFHWR NDGVYPQCCP TQVCDGTD