ID   TX1_OCUSU               Reviewed;          64 AA.
AC   C0HLR8;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-JUN-2021, sequence version 1.
DT   25-MAY-2022, entry version 4.
DE   RecName: Full=Toxin Osu1 {ECO:0000303|PubMed:33597864};
DE   AltName: Full=Kappa-lycotoxin-Os1a {ECO:0000305};
DE            Short=Kappa-LCTX-Os1a {ECO:0000305};
OS   Oculicosa supermirabilis (Central Asian wolf-spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Lycosoidea; Lycosidae; Oculicosa.
OX   NCBI_TaxID=2739307;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY,
RP   RECOMBINANT EXPRESSION, PROBABLE DISULFIDE BONDS, 3D-STRUCTURE MODELING,
RP   AND TOXIC DOSE.
RC   TISSUE=Venom;
RX   PubMed=33597864; DOI=10.3389/fphar.2020.563858;
RA   Alvarado D., Cardoso-Arenas S., Corrales-Garcia L.L., Clement H.,
RA   Arenas I., Montero-Dominguez P.A., Olamendi-Portugal T., Zamudio F.,
RA   Csoti A., Borrego J., Panyi G., Papp F., Corzo G.;
RT   "A novel insecticidal spider peptide that affects the mammalian voltage-
RT   gated ion channel hKv1.5.";
RL   Front. Pharmacol. 11:563858-563858(2020).
CC   -!- FUNCTION: Insecticidal to house crickets (PubMed:33597864). It induces
CC       an excitatory slow-onset impact that leads to irreversible spastic
CC       paralysis (PubMed:33597864). It also modifies human voltage-gated
CC       potassium channel Kv1.5/KCNA5 (PubMed:33597864). Most likely, it binds
CC       to the voltage-sensing domain of the channel, suggesting it does not
CC       block the pore but prevents its opening at physiological membrane
CC       potentials (PubMed:33597864). The recombinant peptide binds to the
CC       channel in an irreversible manner and slows down the hKv1.5 current
CC       activation kinetics (PubMed:33597864). It is not toxic to mice, when
CC       intracranially injected (at 0.5 ug/g mouse) (PubMed:33597864).
CC       {ECO:0000269|PubMed:33597864}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:33597864}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:33597864}.
CC   -!- MASS SPECTROMETRY: Mass=7477.4; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:33597864};
CC   -!- TOXIC DOSE: PD(50) is 0.05 ug/g (6.6 pmol/g) in house crickets (Acheta
CC       domesticus). {ECO:0000269|PubMed:33597864}.
CC   -!- TOXIC DOSE: LD(50) is <0.1 ug/g (<13.3 pmol/g) in house crickets
CC       (Acheta domesticus). {ECO:0000269|PubMed:33597864}.
CC   -!- MISCELLANEOUS: This toxin represents a concentration of ~60 ug per mg
CC       of O.supermirabilis dry crude venom. {ECO:0000269|PubMed:33597864}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 04 (omega-agtx) family. 01 (type
CC       I omega-agtx) subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; C0HLR8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR013605; Toxin_34.
DR   Pfam; PF08396; Toxin_34; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Neurotoxin; Potassium channel impairing toxin; Secreted; Toxin;
KW   Voltage-gated potassium channel impairing toxin.
FT   CHAIN           1..64
FT                   /note="Toxin Osu1"
FT                   /evidence="ECO:0000269|PubMed:33597864"
FT                   /id="PRO_0000453031"
FT   DISULFID        10..26
FT                   /evidence="ECO:0000305|PubMed:33597864"
FT   DISULFID        17..56
FT                   /evidence="ECO:0000305|PubMed:33597864"
FT   DISULFID        19..42
FT                   /evidence="ECO:0000305|PubMed:33597864"
FT   DISULFID        28..40
FT                   /evidence="ECO:0000305|PubMed:33597864"
FT   UNSURE          53
FT                   /note="Assigned by comparison with orthologs and mass
FT                   difference"
FT                   /evidence="ECO:0000305|PubMed:33597864"
SQ   SEQUENCE   64 AA;  7486 MW;  7C1AA0A38A614419 CRC64;
     RLALPPGAVC NGHKSDCQCF GAKYKCSCPF FWRFRKSAEC HCKKGWAWTA IKKRSCHNRY
     QWSD