ID   TX20A_AGEAP             Reviewed;          48 AA.
AC   P0DL48;
DT   24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT   24-JUN-2015, sequence version 1.
DT   25-MAY-2022, entry version 16.
DE   RecName: Full=Omega-agatoxin-Aa5a {ECO:0000305};
DE            Short=Omega-AGTX-Aa5a {ECO:0000305};
DE   AltName: Full=Peptide J2 {ECO:0000303|Ref.1};
OS   Agelenopsis aperta (North American funnel-web spider) (Agelenopsis
OS   gertschi).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Agelenidae; Agelenopsis.
OX   NCBI_TaxID=6908;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RA   Phillips D., Kelly M.E., Saccomano N.A., Volkmann R.A.;
RT   "Calcium channel blocking polypeptide from agelenopsis aperta and
RT   therapeutic methods employing it.";
RL   Patent number US5599559, 04-FEB-1997.
CC   -!- FUNCTION: The toxin blocks voltage-gated calcium channels in rat
CC       cerebellar granule cells (IC(50)=200 nM). {ECO:0000269|Ref.1}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|Ref.1}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:P30288}.
CC   -!- MASS SPECTROMETRY: Mass=5474; Method=Unknown; Note=Average mass.;
CC       Evidence={ECO:0000269|Ref.1};
CC   -!- SIMILARITY: Belongs to the neurotoxin 02 (plectoxin) family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P0DL48; -.
DR   SMR; P0DL48; -.
DR   PRIDE; P0DL48; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004169; Spidertoxin.
DR   Pfam; PF02819; Toxin_9; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated calcium channel impairing toxin.
FT   CHAIN           1..48
FT                   /note="Omega-agatoxin-Aa5a"
FT                   /evidence="ECO:0000269|Ref.1"
FT                   /id="PRO_0000433343"
FT   DISULFID        3..16
FT                   /evidence="ECO:0000250|UniProtKB:P30288"
FT   DISULFID        10..21
FT                   /evidence="ECO:0000250|UniProtKB:P30288"
FT   DISULFID        15..32
FT                   /evidence="ECO:0000250|UniProtKB:P30288"
FT   DISULFID        23..30
FT                   /evidence="ECO:0000250|UniProtKB:P30288"
SQ   SEQUENCE   48 AA;  5485 MW;  7DF62D82967018E8 CRC64;
     EACAGAYKSC DKVKCCHDRR CRCNIAMDNC VCKLFYCELF GTCDRLKP