ID   TX20A_MANRB             Reviewed;          60 AA.
AC   A0A6G9KIR5;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   12-AUG-2020, sequence version 1.
DT   25-MAY-2022, entry version 6.
DE   RecName: Full=U20-myrmicitoxin-Mri1a {ECO:0000303|PubMed:32182430};
DE            Short=U20-MYRTX-Mri1a {ECO:0000303|PubMed:32182430};
DE   Flags: Precursor;
OS   Manica rubida (European giant red ant).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC   Formicidae; Myrmicinae; Manica.
OX   NCBI_TaxID=219785 {ECO:0000312|EMBL:QIQ51453.1};
RN   [1] {ECO:0000312|EMBL:QIQ51453.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 35-59, FUNCTION,
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, AND TOXIC
RP   DOSE.
RC   TISSUE=Venom gland {ECO:0000312|EMBL:QIQ51453.1};
RX   PubMed=32182430; DOI=10.1021/acs.jproteome.0c00048;
RA   Touchard A., Aili S.R., Tene N., Barasse V., Klopp C., Dejean A.,
RA   Kini R.M., Mrinalini X., Coquet L., Jouenne T., Lefranc B., Leprince J.,
RA   Escoubas P., Nicholson G.M., Treilhou M., Bonnafe E.;
RT   "Venom Peptide Repertoire of the European Myrmicine Ant Manica rubida:
RT   Identification of Insecticidal Toxins.";
RL   J. Proteome Res. 19:1800-1811(2020).
CC   -!- FUNCTION: Induces paralysis 5 minutes after injection into blowflies
CC       (L.caesar), and then death within 24 hours (PubMed:32182430). May have
CC       antimicrobial properties, like most ant linear peptides (By
CC       similarity). {ECO:0000250|UniProtKB:P0DSJ5,
CC       ECO:0000269|PubMed:32182430}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:32182430}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:32182430}.
CC   -!- MASS SPECTROMETRY: Mass=2739.6; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:32182430};
CC   -!- TOXIC DOSE: LD(50) is 75.45 +-2.3 nmol/g in blowfly (L.caesar) (at 24
CC       hours post-injection). {ECO:0000269|PubMed:32182430}.
CC   -!- TOXIC DOSE: PD(50) is 69.16 +-8.6 nmol/g in blowfly (L.caesar) (at 1
CC       hour post-injection). {ECO:0000269|PubMed:32182430}.
CC   -!- TOXIC DOSE: PD(50) is 74.67 +-21 nmol/g in blowfly (L.caesar) (at 24
CC       hours post-injection). {ECO:0000269|PubMed:32182430}.
CC   -!- SIMILARITY: Belongs to the formicidae venom precursor-01 superfamily.
CC       {ECO:0000305}.
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DR   EMBL; MN765043; QIQ51453.1; -; mRNA.
DR   AlphaFoldDB; A0A6G9KIR5; -.
DR   SMR; A0A6G9KIR5; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0035738; P:envenomation resulting in modulation of process in another organism; IDA:UniProtKB.
DR   GO; GO:0035821; P:modulation of process of another organism; IDA:UniProtKB.
DR   GO; GO:0044616; P:modulation of relaxation of muscle in another organism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Secreted; Signal; Toxin.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PROPEP          25..34
FT                   /evidence="ECO:0000305|PubMed:32182430"
FT                   /id="PRO_0000453053"
FT   PEPTIDE         35..60
FT                   /note="U20-myrmicitoxin-Mri1a"
FT                   /evidence="ECO:0000269|PubMed:32182430"
FT                   /id="PRO_0000453054"
SQ   SEQUENCE   60 AA;  6276 MW;  BA596D6D01A3C0DE CRC64;
     MKSVILLFAV IAIIVAVIIP AINGESSSNP SANAGIMESL KQLSAKAEEL IKKLLAKKAK