TX20A_PHORI
ID TX20A_PHORI Reviewed; 43 AA.
AC P83911;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2004, sequence version 1.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=Omega-ctenitoxin-Pr1a {ECO:0000305};
DE Short=Omega-CNTX-Pr1a {ECO:0000305};
DE AltName: Full=Neurotoxin PRTx17C3;
DE AltName: Full=Neurotoxin PRTx3-7;
DE Short=Tx3-7;
OS Phoneutria reidyi (Brazilian Amazonian armed spider) (Ctenus reidyi).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Lycosoidea; Ctenidae; Phoneutria.
OX NCBI_TaxID=272752 {ECO:0000305};
RN [1]
RP PROTEIN SEQUENCE, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND AMIDATION AT
RP GLY-43.
RC TISSUE=Venom;
RX PubMed=16278100; DOI=10.1016/j.cbpc.2005.09.010;
RA Richardson M., Pimenta A.M., Bemquerer M.P., Santoro M.M., Beirao P.S.,
RA Lima M.E., Figueiredo S.G., Bloch C. Jr., Vasconcelos E.A., Campos F.A.,
RA Gomes P.C., Cordeiro M.N.;
RT "Comparison of the partial proteomes of the venoms of Brazilian spiders of
RT the genus Phoneutria.";
RL Comp. Biochem. Physiol. 142:173-187(2006).
RN [2]
RP PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AMIDATION AT GLY-43, AND
RP MASS SPECTROMETRY.
RC TISSUE=Venom;
RX PubMed=17151945; DOI=10.1007/s10571-006-9123-z;
RA Vieira L.B., Pimenta A.M.C., Richardson M., Bemquerer M.P., Reis H.J.,
RA Cruz J.S., Gomez M.V., Santoro M.M., Ferreira-de-Oliveira R.,
RA Figueiredo S.G., Snutch T.P., Cordeiro M.N.;
RT "Leftward shift in the voltage-dependence for Ca(2+) currents activation
RT induced by a new toxin from Phoneutria reidyi (Aranae, ctenidae) Venom.";
RL Cell. Mol. Neurobiol. 27:129-146(2007).
CC -!- FUNCTION: Inhibits high-voltage activated calcium channels. Shifts the
CC voltage-dependence for activation towards hyperpolarized membrane
CC potentials for L- (Cav1), P/Q- (Cav2.1/CACNA1A) and R-type
CC (Cav2.3/CACNA1E) calcium currents. Causes immediate agitation and
CC clockwise gyration, followed by the gradual development of general
CC flaccid paralysis when injected intracerebroventricular into mice at
CC dose levels of 5 ug per mouse. {ECO:0000269|PubMed:17151945}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16278100,
CC ECO:0000269|PubMed:17151945}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:16278100, ECO:0000305|PubMed:17151945}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin.
CC {ECO:0000250|UniProtKB:P30288}.
CC -!- MASS SPECTROMETRY: Mass=4627.26; Mass_error=0.3; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:17151945, ECO:0000305};
CC -!- MASS SPECTROMETRY: Mass=4627.9; Method=Unknown;
CC Evidence={ECO:0000269|PubMed:16278100, ECO:0000305};
CC -!- SIMILARITY: Belongs to the neurotoxin 02 (plectoxin) family.
CC {ECO:0000305}.
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DR AlphaFoldDB; P83911; -.
DR SMR; P83911; -.
DR ArachnoServer; AS000270; omega-ctenitoxin-Pr1a.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR CDD; cd12960; Spider_toxin; 1.
DR InterPro; IPR004169; Spidertoxin.
DR Pfam; PF02819; Toxin_9; 1.
PE 1: Evidence at protein level;
KW Amidation; Calcium channel impairing toxin; Direct protein sequencing;
KW Disulfide bond; Ion channel impairing toxin; Knottin; Neurotoxin; Secreted;
KW Toxin; Voltage-gated calcium channel impairing toxin.
FT CHAIN 1..43
FT /note="Omega-ctenitoxin-Pr1a"
FT /evidence="ECO:0000269|PubMed:16278100,
FT ECO:0000269|PubMed:17151945"
FT /id="PRO_0000087639"
FT MOD_RES 43
FT /note="Glycine amide"
FT /evidence="ECO:0000269|PubMed:16278100,
FT ECO:0000269|PubMed:17151945"
FT DISULFID 2..17
FT /evidence="ECO:0000250|UniProtKB:P30288"
FT DISULFID 9..22
FT /evidence="ECO:0000250|UniProtKB:P30288"
FT DISULFID 16..33
FT /evidence="ECO:0000250|UniProtKB:P30288"
FT DISULFID 24..31
FT /evidence="ECO:0000250|UniProtKB:P30288"
SQ SEQUENCE 43 AA; 4640 MW; 52293FC743EFE2D8 CRC64;
ACAGLYKKCG KGVNTCCENR PCKCDLAMGN CICKKKFVEF FGG
