TX211_APOSC
ID TX211_APOSC Reviewed; 74 AA.
AC P49267;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=U1-cyrtautoxin-As1a;
DE Short=U1-CUTX-As1a;
DE AltName: Full=Aptotoxin I;
DE AltName: Full=Aptotoxin-1;
DE AltName: Full=Paralytic peptide I;
DE Short=PP I;
OS Apomastus schlingeri (Trap-door spider) (Aptostichus schlingeri).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Mygalomorphae; Euctenizidae; Apomastus.
OX NCBI_TaxID=12944;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, TOXIC DOSE, BIOASSAY, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RX PubMed=1440641; DOI=10.1016/0041-0101(92)90049-b;
RA Skinner W.S., Dennis P.A., Li J.P., Quistad G.B.;
RT "Identification of insecticidal peptides from venom of the trap-door
RT spider, Aptostichus schlingeri (Ctenizidae).";
RL Toxicon 30:1043-1050(1992).
CC -!- FUNCTION: Neurotoxin with probable ion channel impairing activity. In
CC vivo, is both paralytic and lethal, when injected into lepidopteran
CC larvae. {ECO:0000269|PubMed:1440641}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:1440641}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:1440641}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin.
CC {ECO:0000250|UniProtKB:A0A452CSQ9}.
CC -!- TOXIC DOSE: LD(50) is 1.40 mg/kg by subcutaneous injection.
CC {ECO:0000269|PubMed:1440641}.
CC -!- SIMILARITY: Belongs to the neurotoxin 21 family. {ECO:0000305}.
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DR PIR; F44007; F44007.
DR AlphaFoldDB; P49267; -.
DR SMR; P49267; -.
DR ArachnoServer; AS000396; U1-cyrtautoxin-As1a.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR035311; Cys_Knot_tox.
DR Pfam; PF17486; Cys_Knot_tox; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW Knottin; Neurotoxin; Secreted; Toxin.
FT CHAIN 1..74
FT /note="U1-cyrtautoxin-As1a"
FT /id="PRO_0000087663"
FT DISULFID 23..37
FT /evidence="ECO:0000250|UniProtKB:A0A452CSQ9"
FT DISULFID 30..49
FT /evidence="ECO:0000250|UniProtKB:A0A452CSQ9"
FT DISULFID 36..64
FT /evidence="ECO:0000250|UniProtKB:A0A452CSQ9"
FT DISULFID 67..74
FT /evidence="ECO:0000250|UniProtKB:A0A452CSQ9"
SQ SEQUENCE 74 AA; 8062 MW; 813261466F1D4A7D CRC64;
EIAQNLGSGI PHIRTKLPNG QWCKTPGDLC SSRSECCKAE DSVTYSSGCS RQWSGQQGTF
INQCRTCNVE SSMC