TX219_APOSC
ID TX219_APOSC Reviewed; 76 AA.
AC P49272;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=U1-cyrtautoxin-As1d;
DE Short=U1-CUTX-As1d;
DE AltName: Full=Aptotoxin IX;
DE AltName: Full=Aptotoxin-9;
DE AltName: Full=Paralytic peptide IX;
DE Short=PP IX;
OS Apomastus schlingeri (Trap-door spider) (Aptostichus schlingeri).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Mygalomorphae; Euctenizidae; Apomastus.
OX NCBI_TaxID=12944;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, TOXIC DOSE, SUBCELLULAR LOCATION, AND BIOASSAY.
RC TISSUE=Venom;
RX PubMed=1440641; DOI=10.1016/0041-0101(92)90049-b;
RA Skinner W.S., Dennis P.A., Li J.P., Quistad G.B.;
RT "Identification of insecticidal peptides from venom of the trap-door
RT spider, Aptostichus schlingeri (Ctenizidae).";
RL Toxicon 30:1043-1050(1992).
CC -!- FUNCTION: Neurotoxin with probable ion channel impairing activity. In
CC vivo, is both paralytic and lethal, when injected into lepidopteran
CC larvae. {ECO:0000269|PubMed:1440641}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:1440641}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:1440641}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin.
CC {ECO:0000250|UniProtKB:A0A452CSQ9}.
CC -!- TOXIC DOSE: LD(50) is 0.06 mg/kg by subcutaneous injection.
CC {ECO:0000269|PubMed:1440641}.
CC -!- SIMILARITY: Belongs to the neurotoxin 21 family. {ECO:0000305}.
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DR PIR; A44007; A44007.
DR AlphaFoldDB; P49272; -.
DR SMR; P49272; -.
DR ArachnoServer; AS000410; U1-cyrtautoxin-As1d.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR035311; Cys_Knot_tox.
DR Pfam; PF17486; Cys_Knot_tox; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW Knottin; Neurotoxin; Secreted; Toxin.
FT CHAIN 1..76
FT /note="U1-cyrtautoxin-As1d"
FT /id="PRO_0000087666"
FT DISULFID 23..37
FT /evidence="ECO:0000250|UniProtKB:A0A452CSQ9"
FT DISULFID 30..51
FT /evidence="ECO:0000250|UniProtKB:A0A452CSQ9"
FT DISULFID 36..66
FT /evidence="ECO:0000250|UniProtKB:A0A452CSQ9"
FT DISULFID 69..76
FT /evidence="ECO:0000250|UniProtKB:A0A452CSQ9"
SQ SEQUENCE 76 AA; 8366 MW; 3CF75EDAE7161EFC CRC64;
EIPQNLGSDI PHDIIKLPNG QWCKTPGALC SSRSECCKAK HSDSVTYSSG CSRQWSDQQG
LFINQCRTCN VESSMC