ID   TX22A_PLETR             Reviewed;          44 AA.
AC   P34079;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Omega-plectoxin-Pt1a;
DE            Short=Omega-PLTX-Pt1a;
DE   AltName: Full=PLTX-II;
DE   AltName: Full=Toxin PLTX-2;
OS   Plectreurys tristis (Spider) (Plectreurys bispinosus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Haplogynae; Pholcoidea; Plectreuridae; Plectreurys.
OX   NCBI_TaxID=33319;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, PALMITOYLATION AT THR-44, AND AMIDATION AT
RP   THR-44.
RC   TISSUE=Venom;
RX   PubMed=8413602; DOI=10.1038/365496a0;
RA   Brantona W.D., Rudnick M.S., Zhou Y., Eccleston E.D., Fields G.B.,
RA   Bowers L.D.;
RT   "Fatty acylated toxin structure.";
RL   Nature 365:496-497(1993).
RN   [2]
RP   FUNCTION.
RX   PubMed=2826721; DOI=10.1523/jneurosci.07-12-04195.1987;
RA   Branton W.D., Kolton L., Jan Y.N., Jan L.Y.;
RT   "Neurotoxins from Plectreurys spider venom are potent presynaptic blockers
RT   in Drosophila.";
RL   J. Neurosci. 7:4195-4200(1987).
CC   -!- FUNCTION: Toxin that inhibits presynaptic voltage-gated calcium channel
CC       (Cav) in Drosophila nerve terminals, most likely through specific block
CC       of the Cav2 channel (known as Dmca1A). {ECO:0000269|PubMed:2826721,
CC       ECO:0000269|PubMed:8413602}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- PTM: Contains 5 disulfide bonds. {ECO:0000305}.
CC   -!- PTM: Acylation by palmitate is required for biological activity.
CC       {ECO:0000269|PubMed:8413602}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 02 (plectoxin) family. 02
CC       (plectoxin) subfamily.
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DR   PIR; S38275; S38275.
DR   AlphaFoldDB; P34079; -.
DR   SMR; P34079; -.
DR   ArachnoServer; AS000412; omega-plectoxin-Pt1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Calcium channel impairing toxin; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Knottin; Lipoprotein;
KW   Neurotoxin; Palmitate; Presynaptic neurotoxin; Secreted; Toxin;
KW   Voltage-gated calcium channel impairing toxin.
FT   CHAIN           1..44
FT                   /note="Omega-plectoxin-Pt1a"
FT                   /id="PRO_0000087657"
FT   MOD_RES         44
FT                   /note="Threonine amide"
FT                   /evidence="ECO:0000269|PubMed:8413602"
FT   LIPID           44
FT                   /note="O-palmitoyl threonine"
FT                   /evidence="ECO:0000269|PubMed:8413602"
FT   DISULFID        3..17
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
FT   DISULFID        10..23
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
FT   DISULFID        16..35
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
FT   DISULFID        20..42
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
FT   DISULFID        25..33
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
SQ   SEQUENCE   44 AA;  4881 MW;  BD2CA2ECFAE97EE6 CRC64;
     ADCSATGDTC DHTKKCCDDC YTCRCGTPWG ANCRCDYYKA RCDT