ID   TX22D_PLETR             Reviewed;          45 AA.
AC   P36986;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=U1-plectoxin-Pt1d {ECO:0000305};
DE            Short=U1-PLTX-Pt1d {ECO:0000305};
DE   AltName: Full=Plectoxin XII;
DE            Short=PLT-XII;
DE            Short=PLTXII;
DE   AltName: Full=Plectoxin-12;
DE   Flags: Fragment;
OS   Plectreurys tristis (Spider) (Plectreurys bispinosus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Haplogynae; Pholcoidea; Plectreuridae; Plectreurys.
OX   NCBI_TaxID=33319;
RN   [1]
RP   PROTEIN SEQUENCE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=8157635; DOI=10.1016/s0021-9258(19)78096-x;
RA   Quistad G.B., Skinner W.S.;
RT   "Isolation and sequencing of insecticidal peptides from the primitive
RT   hunting spider, Plectreurys tristis (Simon).";
RL   J. Biol. Chem. 269:11098-11101(1994).
CC   -!- FUNCTION: Potent toxin that may paralyze and/or kill insect pests such
CC       as H.virescens (lepidoptera), S.exigua (beet armyworm) and M.sexta
CC       (tobacco hornworm).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:8157635}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:8157635}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 02 (plectoxin) family. 02
CC       (plectoxin) subfamily. {ECO:0000305}.
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DR   PIR; D53613; D53613.
DR   AlphaFoldDB; P36986; -.
DR   SMR; P36986; -.
DR   ArachnoServer; AS000393; U1-plectoxin-Pt1d.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR036836; Agouti_dom_sf.
DR   InterPro; IPR004169; Spidertoxin.
DR   Pfam; PF02819; Toxin_9; 1.
DR   SUPFAM; SSF57055; SSF57055; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Neurotoxin; Secreted;
KW   Toxin.
FT   CHAIN           1..>45
FT                   /note="U1-plectoxin-Pt1d"
FT                   /evidence="ECO:0000269|PubMed:8157635"
FT                   /id="PRO_0000087658"
FT   DISULFID        4..18
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
FT   DISULFID        11..24
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
FT   DISULFID        17..35
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
FT   DISULFID        21..44
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
FT   DISULFID        26..33
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
FT   NON_TER         45
SQ   SEQUENCE   45 AA;  4996 MW;  265ADB501AB2FAC5 CRC64;
     AVKCIGWQET CNGNLPCCNE CVMCECNIMG QNCRCNHPKA TNECE