ID   TX22G_PLETR             Reviewed;          82 AA.
AC   P36983;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=U1-plectoxin-Pt1a {ECO:0000305};
DE            Short=U1-PLTX-Pt1a {ECO:0000305};
DE   AltName: Full=PLT-V/PLT-VI;
DE            Short=PLTVI;
DE   AltName: Full=Plectoxin V/VI;
DE   AltName: Full=Plectoxin-5/6;
DE   Flags: Precursor;
OS   Plectreurys tristis (Spider) (Plectreurys bispinosus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Haplogynae; Pholcoidea; Plectreuridae; Plectreurys.
OX   NCBI_TaxID=33319;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=8763160; DOI=10.1016/0965-1748(95)00070-4;
RA   Leisy D.J., Mattson J.D., Quistad G.B., Kramer S.J., van Beek N.,
RA   Tsai L.W., Enderlin F.E., Woodworth A.R., Digan M.E.;
RT   "Molecular cloning and sequencing of cDNAs encoding insecticidal peptides
RT   from the primitive hunting spider, Plectreurys tristis (Simon).";
RL   Insect Biochem. Mol. Biol. 26:411-417(1996).
RN   [2]
RP   PROTEIN SEQUENCE OF 34-79, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=8157635; DOI=10.1016/s0021-9258(19)78096-x;
RA   Quistad G.B., Skinner W.S.;
RT   "Isolation and sequencing of insecticidal peptides from the primitive
RT   hunting spider, Plectreurys tristis (Simon).";
RL   J. Biol. Chem. 269:11098-11101(1994).
CC   -!- FUNCTION: Potent toxin that may paralyze and/or kill insect pests such
CC       as H.virescens (lepidoptera), S.exigua (beet armyworm) and M.sexta
CC       (tobacco hornworm).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:8157635}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:8157635}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- PTM: Plectoxin-5 presumably undergoes post-translational modification
CC       to give rise to plectoxin-6.
CC   -!- SIMILARITY: Belongs to the neurotoxin 02 (plectoxin) family. 02
CC       (plectoxin) subfamily. {ECO:0000305}.
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DR   EMBL; U29269; AAC47200.1; -; mRNA.
DR   EMBL; U29271; AAC47202.1; -; mRNA.
DR   EMBL; U29272; AAC47203.1; -; mRNA.
DR   EMBL; U29273; AAC47204.1; -; mRNA.
DR   EMBL; U29274; AAC47205.1; -; mRNA.
DR   EMBL; U29275; AAC47206.1; -; mRNA.
DR   EMBL; U29276; AAC47207.1; -; mRNA.
DR   PIR; A53613; A53613.
DR   AlphaFoldDB; P36983; -.
DR   SMR; P36983; -.
DR   ArachnoServer; AS000405; U1-plectoxin-Pt1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004169; Spidertoxin.
DR   Pfam; PF02819; Toxin_9; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Lipoprotein;
KW   Neurotoxin; Palmitate; Secreted; Signal; Toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..33
FT                   /evidence="ECO:0000269|PubMed:8157635"
FT                   /id="PRO_0000035533"
FT   CHAIN           34..79
FT                   /note="U1-plectoxin-Pt1a"
FT                   /evidence="ECO:0000269|PubMed:8157635"
FT                   /id="PRO_0000035534"
FT   PROPEP          80..82
FT                   /id="PRO_0000035535"
FT   LIPID           79
FT                   /note="O-palmitoyl serine"
FT                   /evidence="ECO:0000250|UniProtKB:P34079"
FT   DISULFID        37..51
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
FT   DISULFID        44..57
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
FT   DISULFID        50..68
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
FT   DISULFID        54..77
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
FT   DISULFID        59..66
FT                   /evidence="ECO:0000250|UniProtKB:P83559"
FT   VARIANT         6..7
FT                   /note="FS -> LA (in clones PSCI267, PSCI268, PSCI270 and
FT                   PSCI272)"
FT   VARIANT         11
FT                   /note="V -> I (in clones PSCI268, PSCI270 and PSCI272)"
FT   VARIANT         19
FT                   /note="F -> S (in clones PSCI268, PSCI270 and PSCI272)"
SQ   SEQUENCE   82 AA;  9241 MW;  F744A5703BC77965 CRC64;
     MKHLIFSSAL VCALVVCTFA EEQVNVPFLP DERAVKCIGW QETCNGNLPC CNECVMCECN
     IMGQNCRCNH PKATNECESR RR