TX2B_MYRPI
ID TX2B_MYRPI Reviewed; 23 AA.
AC P0C023;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2005, sequence version 1.
DT 25-MAY-2022, entry version 31.
DE RecName: Full=M-myrmeciitoxin-Mp2b {ECO:0000303|PubMed:26805882};
DE Short=M-MIITX-Mp2b {ECO:0000303|PubMed:26805882};
DE AltName: Full=DELTA-myrtoxin-Mp1a B chain {ECO:0000303|PubMed:32629771};
DE Short=Mp1a B chain {ECO:0000303|PubMed:32629771};
DE AltName: Full=Pilosin-3 subunit b {ECO:0000305};
DE Short=Pilosulin-3b {ECO:0000303|PubMed:15019477};
OS Myrmecia pilosula (Jack jumper ant) (Australian jumper ant).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC Formicidae; Myrmeciinae; Myrmeciini; Myrmecia.
OX NCBI_TaxID=13618;
RN [1]
RP PROTEIN SEQUENCE, MASS SPECTROMETRY, AMIDATION AT GLN-23, SUBUNIT,
RP SUBCELLULAR LOCATION, AND DISULFIDE BOND.
RC TISSUE=Venom;
RX PubMed=15019477; DOI=10.1016/j.toxicon.2003.11.021;
RA Davies N.W., Wiese M.D., Brown S.G.A.;
RT "Characterisation of major peptides in 'jack jumper' ant venom by mass
RT spectrometry.";
RL Toxicon 43:173-183(2004).
RN [2]
RP REVIEW, AND NOMENCLATURE.
RX PubMed=26805882; DOI=10.3390/toxins8010030;
RA Touchard A., Aili S.R., Fox E.G., Escoubas P., Orivel J., Nicholson G.M.,
RA Dejean A.;
RT "The biochemical toxin arsenal from ant venoms.";
RL Toxins 8:1-28(2016).
RN [3]
RP FUNCTION, SYNTHESIS OF 49-74 IN COMPLEX WITH A CHAIN, BIOASSAY, AND TOXIC
RP DOSE.
RX PubMed=32629771; DOI=10.3390/biomedicines8070185;
RA Nixon S.A., Dekan Z., Robinson S.D., Guo S., Vetter I., Kotze A.C.,
RA Alewood P.F., King G.F., Herzig V.;
RT "It takes two: dimerization is essential for the broad-spectrum predatory
RT and defensive activities of the venom peptide Mp1a from the jack jumper ant
RT Myrmecia pilosula.";
RL Biomedicines 8:0-0(2020).
RN [4]
RP STRUCTURE BY NMR IN COMPLEX WITH A CHAIN, FUNCTION, SYNTHESIS OF COMPLEX
RP WITH A CHAIN, AND BIOASSAY.
RX PubMed=28513074; DOI=10.1002/anie.201703360;
RA Dekan Z., Headey S.J., Scanlon M., Baldo B.A., Lee T.H., Aguilar M.I.,
RA Deuis J.R., Vetter I., Elliott A.G., Amado M., Cooper M.A., Alewood D.,
RA Alewood P.F.;
RT "Delta-myrtoxin-Mp1a is a helical heterodimer from the venom of the jack
RT jumper ant that has antimicrobial, membrane-disrupting, and nociceptive
RT activities.";
RL Angew. Chem. Int. Ed. 56:8495-8499(2017).
CC -!- FUNCTION: Heterodimer protein that may serve both defensive (pain-
CC inducing) and predatory (insecticidal) roles (PubMed:28513074). Has
CC membrane-disrupting activity and shows induction of non-specific
CC calcium influx into cells, (PubMed:28513074). Shows broad-spectrum
CC activity against a diverse range of bacteria, and cell lines, as well
CC as hemolytic activity (EC(50)=2.18 uM) (PubMed:28513074). In vivo,
CC shows moderate insecticidal activity against D.melanogaster and potent
CC anthelmintic activity against the veterinary nematode H.contortus
CC (PubMed:32629771). In addition, intraplantar injection into mice
CC induces nocifensive behavior and mechanical allodynia
CC (PubMed:28513074). {ECO:0000269|PubMed:28513074,
CC ECO:0000269|PubMed:32629771}.
CC -!- SUBUNIT: Heterodimer with M-MIITX-Mp2a (pilosulin-3a) (AC Q26464);
CC disulfide-linked (PubMed:15019477). Only heterodimers (and not
CC monomers) have been identified in the venom (PubMed:15019477).
CC {ECO:0000269|PubMed:15019477}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15019477}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:15019477}.
CC -!- MASS SPECTROMETRY: Mass=2457; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:15019477};
CC -!- ALLERGEN: The heterodimer causes an allergic reaction in human. Binds
CC to IgE. It is speculated that the antigenic site is on the A chain.
CC {ECO:0000269|PubMed:15019477}.
CC -!- TOXIC DOSE: LD(50) of the heterodimer is 260.1 +- 16.6 pmol/g towards
CC D.melanogaster. {ECO:0000269|PubMed:32629771}.
CC -!- MISCELLANEOUS: Dekan et al., 2017 and Nixon et al., 2020 report
CC synthesis and functional analysis of heterodimers with different
CC disulfide patterns as well as different A and B chain monomeric forms.
CC {ECO:0000305|PubMed:28513074, ECO:0000305|PubMed:32629771}.
CC -!- SIMILARITY: Belongs to the formicidae venom precursor-01 superfamily.
CC Ant pilosulin family. {ECO:0000305}.
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DR AlphaFoldDB; P0C023; -.
DR Allergome; 3381; Myr p 2.0101.
DR Allergome; 3575; Myr p 2.0102.
DR Allergome; 481; Myr p 2.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Allergen; Amidation; Antibiotic; Antimicrobial; Cytolysis;
KW Direct protein sequencing; Disulfide bond; Hemolysis; Secreted; Toxin.
FT PEPTIDE 1..23
FT /note="M-myrmeciitoxin-Mp2b"
FT /evidence="ECO:0000269|PubMed:15019477"
FT /id="PRO_0000044533"
FT MOD_RES 23
FT /note="Glutamine amide"
FT /evidence="ECO:0000269|PubMed:15019477"
FT DISULFID 10
FT /note="Interchain (with C-71 in M-MIITX-Mp2a)"
FT /evidence="ECO:0000269|PubMed:15019477"
FT DISULFID 17
FT /note="Interchain (with C-64 in M-MIITX-Mp2a)"
FT /evidence="ECO:0000269|PubMed:15019477"
SQ SEQUENCE 23 AA; 2458 MW; 02DCABBDC40A026B CRC64;
LIGLVSKGTC VLVKTVCKKV LKQ
