ID   TX2_HETPN               Reviewed;          74 AA.
AC   P0DV96;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Mu-Sparatoxin-Hp2 {ECO:0000303|PubMed:35202167};
DE   AltName: Full=HptTx-133 {ECO:0000303|PubMed:35202167};
DE   Flags: Precursor;
OS   Heteropoda pingtungensis (Pingtung huntsman spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Dionycha; Sparassidae; Heteropoda.
OX   NCBI_TaxID=2926465;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AMIDATION AT LEU-73,
RP   SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=35202167; DOI=10.3390/toxins14020140;
RA   Liao Q., Kong X., Luo G., Wu X., Li Y., Liu Q., Tang C., Liu Z.;
RT   "Molecular diversity of peptide toxins in the venom of spider heteropoda
RT   pingtungensis as revealed by cdna library and transcriptome sequencing
RT   analysis.";
RL   Toxins 14:0-0(2022).
CC   -!- FUNCTION: Weakly nhibits voltage-gated sodium channels Nav1.7/SCN9A.
CC       High concentration of the toxin (3 uM) inhibits Nav1.7/SCN9A currents
CC       by 80%. {ECO:0000269|PubMed:35202167}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:35202167}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:35202167}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=4561.93; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:35202167};
CC   -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family.
CC       {ECO:0000305}.
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PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Secreted; Signal; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..35
FT                   /evidence="ECO:0000305|PubMed:35202167"
FT                   /id="PRO_0000455815"
FT   CHAIN           36..73
FT                   /note="Mu-Sparatoxin-Hp2"
FT                   /evidence="ECO:0000269|PubMed:35202167"
FT                   /id="PRO_0000455816"
FT   MOD_RES         73
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:35202167"
FT   DISULFID        42..57
FT                   /evidence="ECO:0000250|UniProtKB:P60590"
FT   DISULFID        49..62
FT                   /evidence="ECO:0000250|UniProtKB:P60590"
FT   DISULFID        56..66
FT                   /evidence="ECO:0000250|UniProtKB:P60590"
SQ   SEQUENCE   74 AA;  8467 MW;  93197E3AD2D1E816 CRC64;
     MKIIVLMMML FAAFSAVVLA DKSIEDAALD TVMDRDDDKK ECIGHMGWCA WTDGECCEGY
     RCKLWCRKII DWLG