TX2_LOXIN
ID TX2_LOXIN Reviewed; 105 AA.
AC Q6B4T4;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 25-MAY-2022, entry version 41.
DE RecName: Full=U1-sicaritoxin-Li1b;
DE Short=U1-SCRTX-Li1b;
DE AltName: Full=LiTx2 {ECO:0000303|PubMed:15302533};
DE Flags: Precursor;
OS Loxosceles intermedia (Brown spider).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Haplogynae; Scytodoidea; Sicariidae; Loxosceles.
OX NCBI_TaxID=58218;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 37-53, AND SUBCELLULAR
RP LOCATION.
RC TISSUE=Venom, and Venom gland;
RX PubMed=15302533; DOI=10.1016/j.toxicon.2004.05.028;
RA de Castro C.S., Silvestre F.G., Araujo S.C., Yazbeck G.M., Mangili O.C.,
RA Cruz I., Chavez-Olortegui C., Kalapothakis E.;
RT "Identification and molecular cloning of insecticidal toxins from the venom
RT of the brown spider Loxosceles intermedia.";
RL Toxicon 44:273-280(2004).
CC -!- FUNCTION: Toxin active against insects (S.frugiperda larvae). May act
CC on sodium (Nav) or calcium (Cav) channels.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15302533}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:15302533}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the neurotoxin 28 (Litx) family. {ECO:0000305}.
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DR EMBL; AY681976; AAT85611.1; -; mRNA.
DR AlphaFoldDB; Q6B4T4; -.
DR SMR; Q6B4T4; -.
DR ArachnoServer; AS000251; U1-sicaritoxin-Li1b.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Disulfide bond; Ion channel impairing toxin; Knottin; Neurotoxin; Secreted;
KW Signal; Toxin.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..36
FT /evidence="ECO:0000269|PubMed:15302533"
FT /id="PRO_0000262658"
FT CHAIN 37..82
FT /note="U1-sicaritoxin-Li1b"
FT /id="PRO_0000262659"
FT PROPEP 86..105
FT /id="PRO_0000262660"
FT MOD_RES 82
FT /note="Arginine amide"
FT /evidence="ECO:0000255"
FT DISULFID 38..55
FT /evidence="ECO:0000250"
FT DISULFID 46..60
FT /evidence="ECO:0000250"
FT DISULFID 54..73
FT /evidence="ECO:0000250"
FT DISULFID 62..71
FT /evidence="ECO:0000250"
FT CONFLICT 38
FT /note="C -> G (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 53
FT /note="W -> D (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 105 AA; 12054 MW; 0FB8D28E1CC30174 CRC64;
MKLLFEGLLV LVLIAFVVAE FESDAEKWEA LITQERACKG EGVKGCYYEA DDWCCKKTPC
KCPAWSHERE CRCTQPCNPS CRGKRALMVD PETHRMLSLH RLSEE