ID   TX305_LYCSI             Reviewed;         115 AA.
AC   B6DCQ0;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=U3-lycotoxin-Ls1a;
DE   AltName: Full=Toxin-like structure LSTX-B5;
DE   Flags: Precursor;
OS   Lycosa singoriensis (Wolf spider) (Aranea singoriensis).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Lycosoidea; Lycosidae; Lycosa.
OX   NCBI_TaxID=434756;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=19875276; DOI=10.1016/j.zool.2009.04.001;
RA   Zhang Y., Chen J., Tang X., Wang F., Jiang L., Xiong X., Wang M., Rong M.,
RA   Liu Z., Liang S.;
RT   "Transcriptome analysis of the venom glands of the Chinese wolf spider
RT   Lycosa singoriensis.";
RL   Zoology 113:10-18(2010).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 19 (CSTX) family. 01 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; EU925984; ACI41316.1; -; mRNA.
DR   EMBL; FM863988; CAS03586.1; -; mRNA.
DR   AlphaFoldDB; B6DCQ0; -.
DR   SMR; B6DCQ0; -.
DR   ArachnoServer; AS000938; U3-lycotoxin-Ls1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR019553; Spider_toxin_CSTX_knottin.
DR   InterPro; IPR011142; Spider_toxin_CSTX_Knottin_CS.
DR   Pfam; PF10530; Toxin_35; 1.
DR   PROSITE; PS60029; SPIDER_CSTX; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Knottin; Secreted; Signal; Toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..44
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000401615"
FT   CHAIN           45..115
FT                   /note="U3-lycotoxin-Ls1a"
FT                   /id="PRO_0000401616"
FT   DISULFID        48..63
FT                   /evidence="ECO:0000250"
FT   DISULFID        55..72
FT                   /evidence="ECO:0000250"
FT   DISULFID        62..87
FT                   /evidence="ECO:0000250"
FT   DISULFID        74..85
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   115 AA;  13258 MW;  C2A24A9CAD8EA4A7 CRC64;
     MKFVLLFGVL LVTLFSYSSA EMLDDFDQAD EDELLSSIEK EEARAKECTP RFYDCSHDRH
     SCCRSELFKD VCTCFYPEGG DNEVCTCQQP KHLKYMEKAA DKAKKFGGKI KKWFG