ID   TX30_PHONI              Reviewed;          47 AA.
AC   P83999;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=U18-ctenitoxin-Pn1a;
DE            Short=U18-CNTX-Pn1a;
DE   AltName: Full=Neurotoxin PNTx30C3;
DE   Flags: Fragment;
OS   Phoneutria nigriventer (Brazilian armed spider) (Ctenus nigriventer).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Lycosoidea; Ctenidae; Phoneutria.
OX   NCBI_TaxID=6918;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP   MASS SPECTROMETRY.
RC   TISSUE=Venom;
RA   Richardson M., Pimenta A.M.C., Bemquerer M.P., Santoro M.M.,
RA   Figueiredo S.G., Cordeiro M.N.;
RT   "Neurotoxin PNTx30C3 from venom of Brazilian armed spider Phoneutria
RT   nigriventer has sequence similarities with toxins from other spiders.";
RL   Submitted (JUN-2004) to UniProtKB.
CC   -!- FUNCTION: Neurotoxin. Causes spastic paralysis and death in mice by
CC       intracerebroventricular injection at dose levels of 3 ug per mouse.
CC       {ECO:0000269|Ref.1}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|Ref.1}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=7876.6; Method=Electrospray;
CC       Evidence={ECO:0000269|Ref.1};
CC   -!- SIMILARITY: Belongs to the neurotoxin 37 family. {ECO:0000305}.
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DR   AlphaFoldDB; P83999; -.
DR   SMR; P83999; -.
DR   ArachnoServer; AS000254; U18-ctenitoxin-Pn1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR013605; Toxin_34.
DR   Pfam; PF08396; Toxin_34; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Neurotoxin; Secreted;
KW   Toxin.
FT   CHAIN           1..>47
FT                   /note="U18-ctenitoxin-Pn1a"
FT                   /id="PRO_0000087648"
FT   DISULFID        2..16
FT                   /evidence="ECO:0000305"
FT   DISULFID        9..22
FT                   /evidence="ECO:0000305"
FT   DISULFID        13..46
FT                   /evidence="ECO:0000305"
FT   DISULFID        15..34
FT                   /evidence="ECO:0000305"
FT   DISULFID        24..32
FT                   /evidence="ECO:0000305"
FT   NON_TER         47
FT                   /evidence="ECO:0000303|Ref.1"
SQ   SEQUENCE   47 AA;  5206 MW;  96997F08B9C52B4F CRC64;
     SCFEGGKDCK NDCQCCGKWS YCKCPIWGLF GCSCVIGDSM VEVRKCQ