TX31A_SCOMU
ID TX31A_SCOMU Reviewed; 74 AA.
AC I6RU32;
DT 19-FEB-2014, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 1.
DT 25-MAY-2022, entry version 26.
DE RecName: Full=Kappa-scoloptoxin(03)-Ssm1a {ECO:0000303|PubMed:24847043};
DE Short=Kappa-SLPTX(03)-Ssm1a {ECO:0000303|PubMed:24847043};
DE AltName: Full=Kappa-scoloptoxin-Ssm1a {ECO:0000303|PubMed:22595790};
DE Short=Kappa-SLPTX-Ssm1a {ECO:0000303|PubMed:22595790};
DE Flags: Precursor;
OS Scolopendra mutilans (Chinese red-headed centipede) (Scolopendra
OS subspinipes mutilans).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Myriapoda; Chilopoda;
OC Pleurostigmophora; Scolopendromorpha; Scolopendridae; Scolopendra.
OX NCBI_TaxID=2836329;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-74, FUNCTION, BIOASSAY,
RP DISULFIDE BONDS, LETHAL DOSES, MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom, and Venom gland;
RX PubMed=22595790; DOI=10.1074/mcp.m112.018853;
RA Yang S., Liu Z., Xiao Y., Li Y., Rong M., Liang S., Zhang Z., Yu H.,
RA King G.F., Lai R.;
RT "Chemical punch packed in venoms makes centipedes excellent predators.";
RL Mol. Cell. Proteomics 11:640-650(2012).
RN [2]
RP NOMENCLATURE.
RX PubMed=24847043; DOI=10.1093/molbev/msu162;
RA Undheim E.A., Jones A., Clauser K.R., Holland J.W., Pineda S.S., King G.F.,
RA Fry B.G.;
RT "Clawing through evolution: toxin diversification and convergence in the
RT ancient lineage Chilopoda (centipedes).";
RL Mol. Biol. Evol. 31:2124-2148(2014).
CC -!- FUNCTION: This toxin inhibits voltage-gated potassium channel currents
CC in DRG neurons (IC(50)=44.2 nM). In vivo, insects injected with this
CC toxin showed signs of neurotoxicity including twitching, paralysis, and
CC body contraction. {ECO:0000269|PubMed:22595790}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22595790}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:22595790}.
CC -!- MASS SPECTROMETRY: Mass=6050.2; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:22595790};
CC -!- TOXIC DOSE: the LD(50) ranges from 0.076 mg/kg in adult blowflies to
CC 29.0 mg/kg in mealworms. {ECO:0000269|PubMed:22595790}.
CC -!- SIMILARITY: Belongs to the scoloptoxin-03 family. {ECO:0000305}.
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DR EMBL; JN646114; AFM55003.1; -; mRNA.
DR PDB; 2M35; NMR; -; A=24-74.
DR PDBsum; 2M35; -.
DR AlphaFoldDB; I6RU32; -.
DR BMRB; I6RU32; -.
DR SMR; I6RU32; -.
DR TCDB; 8.B.26.1.1; the scorpion toxin, scoloptoxin (scoloptoxin) family.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Disulfide bond;
KW Ion channel impairing toxin; Neurotoxin; Potassium channel impairing toxin;
KW Secreted; Signal; Toxin; Voltage-gated potassium channel impairing toxin.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:22595790"
FT CHAIN 24..74
FT /note="Kappa-scoloptoxin(03)-Ssm1a"
FT /evidence="ECO:0000269|PubMed:22595790"
FT /id="PRO_0000425469"
FT DISULFID 32..59
FT /evidence="ECO:0000269|PubMed:22595790,
FT ECO:0007744|PDB:2M35"
FT DISULFID 42..58
FT /evidence="ECO:0000269|PubMed:22595790,
FT ECO:0007744|PDB:2M35"
FT DISULFID 45..68
FT /evidence="ECO:0000269|PubMed:22595790,
FT ECO:0007744|PDB:2M35"
FT TURN 27..29
FT /evidence="ECO:0007829|PDB:2M35"
FT TURN 31..33
FT /evidence="ECO:0007829|PDB:2M35"
FT HELIX 36..49
FT /evidence="ECO:0007829|PDB:2M35"
FT HELIX 54..59
FT /evidence="ECO:0007829|PDB:2M35"
FT HELIX 62..72
FT /evidence="ECO:0007829|PDB:2M35"
SQ SEQUENCE 74 AA; 8557 MW; 5A7605F345DD1A1E CRC64;
MNSSIAILLV MALIMFSLDK SYSTDDESGN KCAKTKRREN VCRVCGNRSG NDEYYSECCE
SDYRYHRCLD LLRN
