ID   TX32B_CHIGU             Reviewed;         116 AA.
AC   B1P1I2;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=U30-theraphotoxin-Cg1b;
DE            Short=U30-TRTX-Cg1b;
DE   AltName: Full=Jingzhaotoxin-63;
DE            Short=JZTX-63;
DE   AltName: Full=Peptide F5-9.19;
DE   Flags: Precursor;
OS   Chilobrachys guangxiensis (Chinese earth tiger tarantula) (Chilobrachys
OS   jingzhao).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Chilobrachys.
OX   NCBI_TaxID=278060;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=18581053; DOI=10.1007/s00018-008-8135-x;
RA   Chen J., Deng M., He Q., Meng E., Jiang L., Liao Z., Rong M., Liang S.;
RT   "Molecular diversity and evolution of cystine knot toxins of the tarantula
RT   Chilobrachys jingzhao.";
RL   Cell. Mol. Life Sci. 65:2431-2444(2008).
RN   [2]
RP   PROTEIN SEQUENCE OF 54-65, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=17476710; DOI=10.1002/pmic.200600785;
RA   Liao Z., Cao J., Li S., Yan X., Hu W., He Q., Chen J., Tang J., Xie J.,
RA   Liang S.;
RT   "Proteomic and peptidomic analysis of the venom from Chinese tarantula
RT   Chilobrachys jingzhao.";
RL   Proteomics 7:1892-1907(2007).
CC   -!- FUNCTION: Probable ion channel inhibitor.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 03 (Tx2) family. 02 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; EU233913; ABY71732.1; -; mRNA.
DR   AlphaFoldDB; B1P1I2; -.
DR   ArachnoServer; AS000861; U30-theraphotoxin-Cg1b.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004214; Conotoxin.
DR   Pfam; PF02950; Conotoxin; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Secreted; Signal; Toxin.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   PROPEP          18..53
FT                   /evidence="ECO:0000269|PubMed:17476710"
FT                   /id="PRO_0000398540"
FT   PEPTIDE         54..116
FT                   /note="U30-theraphotoxin-Cg1b"
FT                   /id="PRO_0000398541"
FT   REGION          25..45
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        55..69
FT                   /evidence="ECO:0000305"
FT   DISULFID        62..75
FT                   /evidence="ECO:0000305"
FT   DISULFID        66..112
FT                   /evidence="ECO:0000305"
FT   DISULFID        68..88
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   116 AA;  12733 MW;  8C0CD7D1627D6B4F CRC64;
     MKLCVLTIAT LLVTATSLET QKEIAEGNEL TREETPSLVE HKEDEAAAAS EKRSCIEEWK
     TCENSCECCG SSTICSSTWA EGKEIKLCKN EGGTFKKVLH FIQKGISKLK SCKEGN