ID   TX32_LOXIN              Reviewed;          86 AA.
AC   P0DMD8;
DT   19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT   19-MAR-2014, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=U2-sicaritoxin-Li1b;
DE            Short=U2-SCRTX-Li1b;
DE   Flags: Precursor;
OS   Loxosceles intermedia (Brown spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Haplogynae; Scytodoidea; Sicariidae; Loxosceles.
OX   NCBI_TaxID=58218;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=23751278; DOI=10.1016/j.toxicon.2013.05.014;
RA   Matsubara F.H., Gremski L.H., Meissner G.O., Constantino Lopes E.S.,
RA   Gremski W., Senff-Ribeiro A., Chaim O.M., Veiga S.S.;
RT   "A novel ICK peptide from the Loxosceles intermedia (brown spider) venom
RT   gland: cloning, heterologous expression and immunological cross-reactivity
RT   approaches.";
RL   Toxicon 71:147-158(2013).
CC   -!- FUNCTION: Toxin active against S.frugiperda larvae. May act on sodium
CC       channels (Nav) (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 39 family. {ECO:0000305}.
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DR   AlphaFoldDB; P0DMD8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Amidation; Disulfide bond; Ion channel impairing toxin; Knottin;
KW   Neurotoxin; Secreted; Signal; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..33
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000425846"
FT   CHAIN           34..85
FT                   /note="U2-sicaritoxin-Li1b"
FT                   /id="PRO_0000425847"
FT   MOD_RES         85
FT                   /note="Lysine amide"
FT                   /evidence="ECO:0000250"
FT   DISULFID        35..53
FT                   /evidence="ECO:0000250"
FT   DISULFID        42..62
FT                   /evidence="ECO:0000250"
FT   DISULFID        52..71
FT                   /evidence="ECO:0000250"
FT   DISULFID        64..69
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   86 AA;  9348 MW;  8EC473D3A832E3D2 CRC64;
     MKIELFLVVI FALAIHMATA EEVIESDIEP AERGCIKSGQ RCGSPHGLPS NCCDDWKYKG
     RCGCTMGVCT CGKNCPSRGC DYRTKG