ID   TX33A_MACGS             Reviewed;         124 AA.
AC   Q75WG7;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=U13-hexatoxin-Mg1a;
DE            Short=U13-HXTX-Mg1a;
DE   AltName: Full=Neurotoxin magi-12;
DE   Flags: Precursor;
OS   Macrothele gigas (Japanese funnel web spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Macrothelidae; Macrothele.
OX   NCBI_TaxID=223896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=15246762; DOI=10.1016/j.toxicon.2004.05.012;
RA   Satake H., Villegas E., Oshiro N., Terada K., Shinada T., Corzo G.;
RT   "Rapid and efficient identification of cysteine-rich peptides by random
RT   screening of a venom gland cDNA library from the hexathelid spider
RT   Macrothele gigas.";
RL   Toxicon 44:149-156(2004).
CC   -!- FUNCTION: No toxicity is observed upon intracranial injection into mice
CC       and intrathorax injection into crickets. {ECO:0000269|PubMed:15246762}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=7528.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15246762};
CC   -!- SIMILARITY: Belongs to the neurotoxin 03 (Tx2) family. 03 subfamily.
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DR   EMBL; AB121204; BAD13411.1; -; mRNA.
DR   AlphaFoldDB; Q75WG7; -.
DR   ArachnoServer; AS000364; U13-hexatoxin-Mg1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Disulfide bond; Knottin; Secreted;
KW   Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   PROPEP          18..52
FT                   /id="PRO_0000285711"
FT   CHAIN           53..124
FT                   /note="U13-hexatoxin-Mg1a"
FT                   /id="PRO_0000285712"
FT   DISULFID        54..72
FT                   /evidence="ECO:0000305"
FT   DISULFID        65..78
FT                   /evidence="ECO:0000305"
FT   DISULFID        69..116
FT                   /evidence="ECO:0000305"
FT   DISULFID        71..87
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   124 AA;  13188 MW;  AFD966DB73FE124D CRC64;
     MKLSALVFVA SVMLVAASPV KDVEEPVETH LAADLKTIEE LAKYEEAAVQ KRSCIVGSKN
     IGETCVASCQ CCGATVRCIG EGTKGICNNY QTNNILGQIL LYAKDTVVNT AGLLVCAQDL
     SEYE