TX35A_PHORI
ID TX35A_PHORI Reviewed; 40 AA.
AC P0DPG7;
DT 20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2018, sequence version 1.
DT 25-MAY-2022, entry version 10.
DE RecName: Full=Beta/delta-ctenitoxin-Pr1a {ECO:0000305};
DE Short=Beta/delta-CNTX-Pr1a {ECO:0000305};
DE AltName: Full=Beta/delta-PrIT1 {ECO:0000303|PubMed:26220799};
DE Flags: Fragment;
OS Phoneutria reidyi (Brazilian Amazonian armed spider) (Ctenus reidyi).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Lycosoidea; Ctenidae; Phoneutria.
OX NCBI_TaxID=272752;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, TOXIC DOSE, MASS SPECTROMETRY, SUBCELLULAR
RP LOCATION, AND BIOASSAY.
RC TISSUE=Venom;
RX PubMed=26220799; DOI=10.1016/j.toxicon.2015.07.010;
RA de Oliveira L.C., Campos F.V., Figueiredo S.G., Cordeiro M.N., Adaime B.R.,
RA Richardson M., Pimenta A.M., Martin-Eauclaire M.F., Beirao P.S.,
RA De Lima M.E.;
RT "beta/delta-PrIT1, a highly insecticidal toxin from the venom of the
RT Brazilian spider Phoneutria reidyi (F.O. Pickard-Cambridge, 1897).";
RL Toxicon 104:73-82(2015).
CC -!- FUNCTION: Potent insecticidal toxin that binds to two distinct sites in
CC insect sodium channels, with close affinity (Kd1=34.7 pM and Kd2=35.1
CC pM) (PubMed:26220799). Its association is rather fast (1.4 and 8.5
CC minutes, respectively for sites 1 and 2) and its dissociation is a
CC slower process (5.4 and 32.8 minutes, respectively) (PubMed:26220799).
CC On rat brain synaptosomes the toxin partially competes (~30%) with the
CC beta-toxin CssIV, but does not compete with the alpha-toxin AaII, nor
CC with the beta-toxin Ts VII (PubMed:26220799). On cockroach nerve cord
CC synaptosomes, the toxin does not compete with the anti-insect toxin
CC LqqIT1, but it competes with the 'alpha-like' toxin BomIV (IC(50)=80
CC pM) (PubMed:26220799). In cockroach neurons, the toxin inhibits the
CC inactivation of sodium channels and it shifts the sodium channel
CC activation to hyperpolarizing potentials (PubMed:26220799). Hence, it
CC behaves like an 'alpha-like' toxin and binds preferentially to site 3
CC on the insect Nav channel, located on the domain IV (PubMed:26220799).
CC The toxin may also inhibit the N-methyl-D-aspartate (NMDA)-subtype of
CC ionotropic glutamate receptor (GRIN) (By similarity). In vivo, the
CC toxin causes excitatory effects on insects (PubMed:26220799).
CC {ECO:0000250|UniProtKB:P59367, ECO:0000269|PubMed:26220799}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:26220799}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:26220799}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin. {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=5597.86; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:26220799};
CC -!- TOXIC DOSE: LD(50) is 4 nmol/g in flies (M.domestica).
CC {ECO:0000269|PubMed:26220799}.
CC -!- TOXIC DOSE: LD(50) is 230 pmol/g in cockroaches (P.americana).
CC {ECO:0000269|PubMed:26220799}.
CC -!- MISCELLANEOUS: Is not toxic to mice (30 ug per mouse tested by
CC intracerebroventricular injection). {ECO:0000269|PubMed:26220799}.
CC -!- SIMILARITY: Belongs to the neurotoxin 03 (Tx2) family. 05 subfamily.
CC {ECO:0000305}.
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DR AlphaFoldDB; P0DPG7; -.
DR SMR; P0DPG7; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0035792; C:host cell postsynaptic membrane; IEA:UniProtKB-KW.
DR GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW Ionotropic glutamate receptor inhibitor; Knottin; Neurotoxin;
KW Postsynaptic neurotoxin; Secreted; Toxin;
KW Voltage-gated sodium channel impairing toxin.
FT CHAIN 1..>40
FT /note="Beta/delta-ctenitoxin-Pr1a"
FT /evidence="ECO:0000305|PubMed:26220799"
FT /id="PRO_0000444577"
FT DISULFID 1..15
FT /evidence="ECO:0000305"
FT DISULFID 8..21
FT /evidence="ECO:0000305"
FT DISULFID 12..?
FT /evidence="ECO:0000305"
FT DISULFID 14..31
FT /evidence="ECO:0000305"
FT DISULFID 23..29
FT /evidence="ECO:0000305"
FT NON_TER 40
FT /evidence="ECO:0000305"
SQ SEQUENCE 40 AA; 4366 MW; 5EC28322DACFCB4D CRC64;
CGDINAPCQS DCDCCGYSVT CDCYWGNECK CRESNFAIGM
