ID   TX35B_PHONI             Reviewed;          48 AA.
AC   P84034;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Delta-ctenitoxin-Pn1b {ECO:0000305};
DE            Short=Delta-CNTX-Pn1b {ECO:0000305};
DE   AltName: Full=Neurotoxin PnTx4-3 {ECO:0000303|PubMed:14757211};
OS   Phoneutria nigriventer (Brazilian armed spider) (Ctenus nigriventer).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Lycosoidea; Ctenidae; Phoneutria.
OX   NCBI_TaxID=6918;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND
RP   TOXIC DOSE.
RC   TISSUE=Venom;
RX   PubMed=14757211; DOI=10.1016/j.toxicon.2003.10.009;
RA   Oliveira L.C., De Lima M.E., Pimenta A.M.C., Mansuelle P., Rochat H.,
RA   Cordeiro M.N., Richardson M., Figueiredo S.G.;
RT   "PnTx4-3, a new insect toxin from Phoneutria nigriventer venom elicits the
RT   glutamate uptake inhibition exhibited by PhTx4 toxic fraction.";
RL   Toxicon 42:793-800(2003).
RN   [2]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=16278100; DOI=10.1016/j.cbpc.2005.09.010;
RA   Richardson M., Pimenta A.M., Bemquerer M.P., Santoro M.M., Beirao P.S.,
RA   Lima M.E., Figueiredo S.G., Bloch C. Jr., Vasconcelos E.A., Campos F.A.,
RA   Gomes P.C., Cordeiro M.N.;
RT   "Comparison of the partial proteomes of the venoms of Brazilian spiders of
RT   the genus Phoneutria.";
RL   Comp. Biochem. Physiol. 142:173-187(2006).
CC   -!- FUNCTION: Insecticidal neurotoxin that reversibly inhibits the N-
CC       methyl-D-aspartate (NMDA)-subtype of ionotropic glutamate receptor
CC       (GRIN) and inhibits inactivation of insect sodium channels (Nav) (By
CC       similarity). Inhibits glutamate uptake in rat brain synaptosomes
CC       (PubMed:14757211). In vivo, induces immediate excitatory effects when
CC       injected intrathoracically in houseflies and cockroaches
CC       (PubMed:14757211). {ECO:0000250|UniProtKB:P59367,
CC       ECO:0000269|PubMed:14757211}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:14757211,
CC       ECO:0000269|PubMed:16278100}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:14757211, ECO:0000305|PubMed:16278100}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=5199.49; Mass_error=0.64; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:14757211, ECO:0000269|PubMed:16278100};
CC   -!- MASS SPECTROMETRY: Mass=5199.5; Method=Unknown;
CC       Evidence={ECO:0000269|PubMed:14757211, ECO:0000269|PubMed:16278100};
CC   -!- TOXIC DOSE: LD(50) is 20 ng/house fly. {ECO:0000269|PubMed:14757211}.
CC   -!- MISCELLANEOUS: Has no effect when intracerebroventricularly injected
CC       into mice. {ECO:0000269|PubMed:14757211}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 03 (Tx2) family. 05 subfamily.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P84034; -.
DR   ArachnoServer; AS000278; delta-ctenitoxin-Pn1b.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0035792; C:host cell postsynaptic membrane; IEA:UniProtKB-KW.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Ionotropic glutamate receptor inhibitor; Knottin; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..48
FT                   /note="Delta-ctenitoxin-Pn1b"
FT                   /evidence="ECO:0000269|PubMed:14757211,
FT                   ECO:0000269|PubMed:16278100"
FT                   /id="PRO_0000087629"
FT   DISULFID        1..15
FT                   /evidence="ECO:0000305"
FT   DISULFID        8..21
FT                   /evidence="ECO:0000305"
FT   DISULFID        12..48
FT                   /evidence="ECO:0000305"
FT   DISULFID        14..31
FT                   /evidence="ECO:0000305"
FT   DISULFID        23..29
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   48 AA;  5210 MW;  F0CB6CC03DBB7466 CRC64;
     CGDINAACKE DCDCCGYTTA CDCYWSSSCK CREAAIVIYT APKKKLTC