ID   TX35_ACAPA              Reviewed;         103 AA.
AC   P0DV32;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   23-FEB-2022, sequence version 1.
DT   03-AUG-2022, entry version 3.
DE   RecName: Full=Omega toxin Ap5 {ECO:0000303|PubMed:34363923};
DE   Contains:
DE     RecName: Full=Omega toxin Ap3 {ECO:0000303|PubMed:34363923};
DE   Flags: Precursor;
OS   Acanthoscurria paulensis (Brazilian giant black tarantula spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Acanthoscurria.
OX   NCBI_TaxID=1264770;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, MASS
RP   SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=34363923; DOI=10.1016/j.peptides.2021.170622;
RA   Tibery D.V., de Souza A.C.B., Mourao C.B.F., do Nascimento J.M.,
RA   Schwartz E.F.;
RT   "Purification and characterization of peptides Ap2, Ap3 and Ap5 (omega-
RT   toxins) from the venom of the Brazilian tarantula Acanthoscurria
RT   paulensis.";
RL   Peptides 145:170622-170622(2021).
CC   -!- FUNCTION: [Omega toxin Ap3]: Shows a weak inhibition on the voltage-
CC       gated calcium channel Cav2.1/CACNA1A and some voltage-gated sodium
CC       channels (with 1 uM toxin tested: 22.08% inhibition on Cav2.1/CACNA1A,
CC       6.6% on Nav1.1/SCN1A, 4.2% on Nav1.5, and 16% on Nav1.7).
CC       {ECO:0000269|PubMed:34363923}.
CC   -!- FUNCTION: [Omega toxin Ap5]: Shows a weak inhibition on the voltage-
CC       gated calcium channel Cav2.1/CACNA1A (28.06% at 1 uM).
CC       {ECO:0000269|PubMed:34363923}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:34363923}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:34363923}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: [Omega toxin Ap3]: Mass=4883.7; Method=MALDI;
CC       Note=Monoisotopic mass.; Evidence={ECO:0000269|PubMed:34363923};
CC   -!- MASS SPECTROMETRY: [Omega toxin Ap5]: Mass=5454.7; Method=MALDI;
CC       Note=Monoisotopic mass.; Evidence={ECO:0000269|PubMed:34363923};
CC   -!- MISCELLANEOUS: [Omega toxin Ap3]: Does not show activity on
CC       Cav1.2/CACNA1C, and Cav2.2/CACNA1B. {ECO:0000269|PubMed:34363923}.
CC   -!- MISCELLANEOUS: [Omega toxin Ap5]: Does not show activity on
CC       Nav1.1/SCN1A, Nav1.5/SCN5A, Nav1.7/SCN9A, Cav1.2/CACNA1C, and
CC       Cav2.2/CACNA1B. {ECO:0000269|PubMed:34363923}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 14 (magi-1) family. 08 (Ltx-4)
CC       subfamily. {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR012627; Toxin_22.
DR   Pfam; PF08092; Toxin_22; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Secreted; Signal; Toxin; Voltage-gated calcium channel impairing toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..57
FT                   /evidence="ECO:0000305|PubMed:34363923"
FT                   /id="PRO_0000454753"
FT   CHAIN           58..103
FT                   /note="Omega toxin Ap5"
FT                   /evidence="ECO:0000305|PubMed:34363923"
FT                   /id="PRO_0000454754"
FT   CHAIN           58..98
FT                   /note="Omega toxin Ap3"
FT                   /evidence="ECO:0000305|PubMed:34363923"
FT                   /id="PRO_0000454755"
FT   DISULFID        58..73
FT                   /evidence="ECO:0000305|PubMed:34363923"
FT   DISULFID        65..78
FT                   /evidence="ECO:0000305|PubMed:34363923"
FT   DISULFID        72..93
FT                   /evidence="ECO:0000305|PubMed:34363923"
SQ   SEQUENCE   103 AA;  11834 MW;  4FB795BD7D629C76 CRC64;
     MNTIQVILFA VVLVLTVTVG QADEDSAETS LLRKLEEAEA SMFGQYLEES KNSREKRCAV
     ENVPCDKDRP GCCREYECLK PTGYGWWYGS YYCYKKKERL IST