ID   TX36A_ANCSP             Reviewed;          47 AA.
AC   P84028;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Delta-ctenitoxin-Asp2e;
DE            Short=Delta-CNTX-Asp2e;
DE   AltName: Full=Neurotoxin ANC45C1;
OS   Ancylometes sp. (South American fishing spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Lycosoidea; Ctenidae; Ancylometes;
OC   unclassified Ancylometes.
OX   NCBI_TaxID=280265;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom;
RA   Richardson M., Pimenta A.M.C., Bemquerer M.P., Santoro M.M.,
RA   Figueiredo S.G., Cordeiro M.N.;
RT   "Protein ANC45C1 from venom of South American fishing spider (Ancylometes
RT   spp.) has sequence similarities to Na channel antagonists from venoms of
RT   Phoneutria spiders.";
RL   Submitted (JUN-2004) to UniProtKB.
CC   -!- FUNCTION: Inhibits the inactivation of voltage-gated sodium channels
CC       (Nav). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|Ref.1}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=4979.3; Method=Electrospray;
CC       Evidence={ECO:0000269|Ref.1};
CC   -!- SIMILARITY: Belongs to the neurotoxin 03 (Tx2) family. 06 subfamily.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P84028; -.
DR   SMR; P84028; -.
DR   ArachnoServer; AS000016; delta-ctenitoxin-Asp2e.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR035285; CNTX.
DR   Pfam; PF17492; D_CNTX; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..47
FT                   /note="Delta-ctenitoxin-Asp2e"
FT                   /id="PRO_0000087631"
FT   DISULFID        3..17
FT                   /evidence="ECO:0000305"
FT   DISULFID        10..23
FT                   /evidence="ECO:0000305"
FT   DISULFID        14..46
FT                   /evidence="ECO:0000305"
FT   DISULFID        16..31
FT                   /evidence="ECO:0000305"
FT   DISULFID        25..29
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   47 AA;  4988 MW;  2C0D79834CF613C5 CRC64;
     ATCAGQDKPC KVNCDCCGER GECVCGGPCI CRQGNVFIAW SKLMTCK