ID   TX3_PSACA               Reviewed;          34 AA.
AC   P0C246;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2006, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=Tau-theraphotoxin-Pc1c;
DE            Short=Tau-TRTX-Pc1c;
DE   AltName: Full=Vanillotoxin-3 {ECO:0000303|PubMed:17093448};
DE            Short=VaTx3 {ECO:0000303|PubMed:17093448};
OS   Psalmopoeus cambridgei (Trinidad chevron tarantula).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Psalmopoeus.
OX   NCBI_TaxID=179874;
RN   [1]
RP   PROTEIN SEQUENCE, SYNTHESIS, FUNCTION, TOXIN TARGET, MASS SPECTROMETRY,
RP   SUBCELLULAR LOCATION, AND AMIDATION AT PHE-34.
RC   TISSUE=Venom;
RX   PubMed=17093448; DOI=10.1038/nature05285;
RA   Siemens J., Zhou S., Piskorowski R., Nikai T., Lumpkin E.A., Basbaum A.I.,
RA   King D., Julius D.;
RT   "Spider toxins activate the capsaicin receptor to produce inflammatory
RT   pain.";
RL   Nature 444:208-212(2006).
CC   -!- FUNCTION: Selectively activates mammalian TRPV1, or capsaicin receptor,
CC       a non-selective cation channel expressed by sensory neurons of the pain
CC       pathway. Is more potent than VaTx1 and VaTx2. Interacts with distinct
CC       regions of the channel than capsaicin, since it only acts on the
CC       extracellular face of the channel, and capsaicin binds to the cytosolic
CC       side. Also activates avian TRPV1, which is insensitive to capsaicin. In
CC       mice, elicits pain-related behaviors, such as licking and flinching of
CC       the affected limb. The paw of toxin-injected mice shows substantial
CC       edema. {ECO:0000269|PubMed:17093448}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17093448}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:17093448}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:P60992}.
CC   -!- MASS SPECTROMETRY: Mass=4179.0; Method=Unknown;
CC       Evidence={ECO:0000269|PubMed:17093448};
CC   -!- MISCELLANEOUS: This toxin does not affect TRPV2, TRPV3, TRPV4, TRPA1,
CC       TRPM8, Kv1.2/KCNA2, Kv2.1/KCNB1 and Kv4.2/KCND2 potassium channels.
CC       {ECO:0000269|PubMed:17093448}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family. 62 (Vatx)
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0C246; -.
DR   SMR; P0C246; -.
DR   TCDB; 8.B.5.3.2; the na(+)/k(+)/ca(2+) channel targeting tarantula huwentoxin (tht) family.
DR   ArachnoServer; AS000276; tau-theraphotoxin-Pc1c.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011696; Huwentoxin-1.
DR   InterPro; IPR013140; Huwentoxin_CS1.
DR   Pfam; PF07740; Toxin_12; 1.
DR   PROSITE; PS60021; HWTX_1; 1.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin; Secreted; Toxin.
FT   CHAIN           1..34
FT                   /note="Tau-theraphotoxin-Pc1c"
FT                   /evidence="ECO:0000269|PubMed:17093448"
FT                   /id="PRO_0000262453"
FT   MOD_RES         34
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|PubMed:17093448"
FT   DISULFID        2..16
FT                   /evidence="ECO:0000250|UniProtKB:P60992"
FT   DISULFID        9..21
FT                   /evidence="ECO:0000250|UniProtKB:P60992"
FT   DISULFID        15..28
FT                   /evidence="ECO:0000250|UniProtKB:P60992"
SQ   SEQUENCE   34 AA;  4190 MW;  D1220D7C1D716912 CRC64;
     ECRWYLGGCK EDSECCEHLQ CHSYWEWCLW DGSF